Additional file 5.
Multi-gene phylogenetic reconstruction from cDNA (see Methods section: Multi-gene phylogenetic analyses of cDNA sequences) including introgressed sequences. Multi-gene phylogenetic relationships as shown in Figure 1 but adding sequences of F. guiryi from northern Portugal, where the species co-occurs in sympatry with F. vesiculosus and F. spiralis, creating an introgressed range for F. guiryi that continues northwards [11,25,26,47]. Methods are the same as described for Figure 1. Comparison of this tree with Figure 1 illustrates the effect of introgressed contact regions in preventing phylogenetic resolution, by confounding vertical lineage splitting with horizontal introgressive mixing.
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Cánovas et al. BMC Evolutionary Biology 2011 11:371 doi:10.1186/1471-2148-11-371