Expression analysis of 5 Phytophthora parasitica LRR-RK genes during their interaction with Arabidopsis thaliana. The relative mRNA levels have been quantified by qRT-PCR at different development stages: samples corresponding to Phytophthora parasitica mycelium grown in V8 medium (M), Phytophthora parasitica motile zoospores (Z), and Arabidopsis thaliana roots collected 2.5 (At-2.5 hpi), 6 (At-6 hpi), 10.5 (At-10.5 hpi), 30 (At-30 hpi) and 96 (At-96 hpi) hours after inoculation with Phytophthora parasitica zoospores . The data are presented as expression ratios relative to the mean expression values of three reference genes (2-DCT). Two independent RNA extractions corresponding to a pool of more than 5 biological replicates each were used. • and ×, biological replicates; bars, mean values.
Diévart et al. BMC Evolutionary Biology 2011 11:367 doi:10.1186/1471-2148-11-367