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Open Access Highly Accessed Research article

Deep mitochondrial divergence within a Heliconius butterfly species is not explained by cryptic speciation or endosymbiotic bacteria

Astrid G Muñoz1*, Simon W Baxter2, Mauricio Linares13 and Chris D Jiggins2

Author affiliations

1 Instituto de Genética, Departamento de Ciencias Biologicas-Facultad de Ciencias, Universidad de los Andes, Carrera 1 No 18A - 70, P.O.Box 4976, Bogotá D.C., Colombia

2 Department of Zoology, University of Cambridge, Downing Street, Cambridge CB2 3EJ, UK

3 Facultad de Ciencias Naturales y Matemáticas, Universidad del Rosario, Carrera 24 No 63C -69, Bogotá D.C., Colombia

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Citation and License

BMC Evolutionary Biology 2011, 11:358  doi:10.1186/1471-2148-11-358

Published: 12 December 2011

Abstract

Background

Cryptic population structure can be an indicator of incipient speciation or historical processes. We investigated a previously documented deep break in the mitochondrial haplotypes of Heliconius erato chestertonii to explore the possibility of cryptic speciation, and also the possible presence of endosymbiont bacteria that might drive mitochondrial population structure.

Results

Among a sample of 315 individuals from 16 populations of western Colombia, two principal mtDNA clades were detected with 2.15% divergence and we confirmed this structure was weakly associated with geography. The first mtDNA clade included 87% of individuals from northern populations and was the sister group of H. erato members of Andes western, while the second clade contained most individuals from southern populations (78%), which shared haplotypes with an Ecuadorian race of H. erato. In contrast, analysis using AFLP markers showed H. e. chestertonii to be a genetically homogeneous species with no association between mitochondrial divergence and AFLP structure. The lack of congruence between molecular markers suggests that cryptic speciation is not a plausible explanation for the deep mitochondrial divergence in H. e chestertonii. We also carried out the first tests for the presence of endosymbiontic bacteria in Heliconius, and identified two distinct lineages of Wolbachia within H. e. chestertonii. However, neither of the principal mitochondrial clades of H. e. chestertonii was directly associated with the patterns of infection.

Conclusions

We conclude that historical demographic processes are the most likely explanation for the high mitochondrial differentiation in H. e. chestertonii, perhaps due to gene flow between Cauca valley H. e. chestertonii and west Pacific slope populations of H. erato.