Table 3

Phenotypic differences between the three P. hispanicus lineages

a. Loadings

b. Means ± SE per lineage

c. Univariate ANOVAs


Variable

LF1

LF2

edwardsianus lineage

Central lineage

Western lineage

test statistic

Padjusted


Femoral pores (#)

-4.180

0.858

12.1 ± 0.1

9.9 ± 0.1

11.2 ± 0.2

F 2,208 = 102.03

< 0.001

Throat scales (#)

-3.247

-0.165

20.4 ± 0.2

17.9 ± 0.2

18.9 ± 0.3

F 2,208 = 40.55

< 0.001

Ocelli (#)

-2.387

1.473

1.9 ± 0.1

0.7 ± 0.1

2.0 ± 0.4

F 2,208 = 31.71

< 0.001

SVL ratio

-2.262

-0.117

2.492 ± 0.038

2.199 ± 0.039

2.337 ± 0.053

F 2,208 = 12.46

< 0.001

Snout shape

-0.524

1.221

1.065 ± 0.005

1.043 ± 0.006

1.098 ± 0.009

F 2,208 = 12.36

< 0.001

Anal scale width (mm)

-0.304

0.446

0.063 ± 0.001

0.061 ± 0.001

0.066 ± 0.001

F 2,208 = 3.77

0.024

Body mass (g)

1.192

-0.794

1.693 ± 0.03

1.877 ± 0.04

1.866 ± 0.07

F 2,208 = 7.12

0.003

Ventral scales (#)

1.416

-0.741

24.6 ± 0.2

25.8 ± 0.3

25.1 ± 0.4

F 2,208 = 6.55

0.004

Head ratio

1.423

-0.483

0.482 ± 0.003

0.500 ± 0.002

0.498 ± 0.004

F 2,208 = 7.49

0.001

SVL (mm)

2.094

-0.882

46.39 ± 0.33

49.81 ± 0.43

48.31 ± 0.72

F 2,208 = 18.04

0.001

Collar scales (#)

2.225

-1.185

0.3 ± 0.1

1.3 ± 0.2

0.7 ± 0.2

Ha2,208 = 29.25

< 0.001

Nuptial coloration

3.774

1.266

0.5 ± 0.1

4.3 ± 0.3

5.2 ± 0.5

Ha2,208 = 106.59

< 0.001


d.Post-hoc analyses


Compared lineages


Variable

edwardsianus vs Central

edwardsianus vs Western

Central vs Western


Femoral pores (#)

t170 = 9.13, P < 0.0001

t145 = 5.09, P < 0.0001

t101 = 6.82, P < 0.0001

Throat scales (#)

t170 = 9.03, P < 0.0001

t145 = 4.38, P = 0.0002

t101 = 2.92, P = 0.038

Ocelli (#)

t170 = 7.60, P < 0.0001

t145 = 0.22, P = 0.830

t101 = 6.29, P < 0.0001

SVL ratio

t170 = 5.20, P < 0.0001

t145 = 2.33, P = 0.105

t101 = 1.89, P = 0.366

Snout shape

t170 = 2.53, P = 0.024

t145 = 3.28, P = 0.012

t101 = 5.13, P < 0.0001

Anal scale width (mm)

t170 = 1.60, P = 0.111

t145 = 1.62, P = 0.429

t101 = 2.76, P = 0.055

Body mass (g)

t170 = 3.51, P = 0.002

t145 = 2.46, P = 0.105

t101 = 0.47, P = 1.000

Ventral scales (#)

t170 = 3.63, P = 0.002

t145 = 1.24, P = 0.537

t101 = 1.68, P = 0.483

Head ratio

t170 = 3.29, P = 0.004

t145 = 3.01, P = 0.025

t101 = 0.21, P = 1.000

SVL (mm)

t170 = 5.76, P < 0.0001

t145 = 2.43, P = 0.105

t101 = 1.53, P = 0.520

Collar scales (#)

TcBF = 5.50, P < 0.0002

TcBF = 1.79, P = 0.537

TbBF = -2.44, P = 0.322

Nuptial coloration

TcBF = 16.3, P < 0.0003

TcBF = 9.18, P < 0.0001

TbBF = 1.82, P = 0.537


aKruskal Wallis test; bBehrens Fisher test

a. Factor loadings of each linear function (LF) derived from discriminant function analyses. b. Means (± SE) per lineage of the measured traits used for the phenotypic analyses. c. Results of univariate ANOVAs. d. Post-hoc tests allow understanding what lineages differ in what traits. All P-values are adjusted for multiple testing using Bonferroni procedures.

Fitze et al. BMC Evolutionary Biology 2011 11:347   doi:10.1186/1471-2148-11-347

Open Data