Figure 6.

Multiple sequence alignment of Barhl proteins. Conserved domains found in the Barhl proteins are shown. Consensus sequences of the functional domains are in red brackets (A) FIL domain 1 is present in all vertebrates but medaka. (B) FIL domain 2 is shared between all vertebrates, but can only be partially aligned with Xenopus. (C) Homeodomain (conserved DNA binding motif). All Barhl2 proteins have an aspartic acid residue at position 34, an alanine instead of serine at position 38 and an asparagine residue instead of alanine at position 15 (asterisks). The blue background represents the Blosum62 score: high similarities are in dark blue while lower similarities are indicated in light blue.