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Open Access Research article

Evolutionary and functional insights into Leishmania META1: evidence for lateral gene transfer and a role for META1 in secretion

Vidhi Puri1, Aneesh Goyal13, Rajan Sankaranarayanan1, Anton J Enright2 and Tushar Vaidya1*

Author Affiliations

1 Centre for Cellular and Molecular Biology, Council for Scientific and Industrial Research, Uppal Road, Hyderabad - 500 007, Andhra Pradesh, India

2 EMBL-EBI, Wellcome Trust Genome Campus, Hinxton, Cambridge, CB10 1SD, UK

3 Syngene International Limited, Biocon Park, Plot No. 2 & 3, Bommasandra Industrial Area-IV Phase, Bommasandra-Jigani Link Road, Bengaluru- 560099, Karnataka, India

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BMC Evolutionary Biology 2011, 11:334  doi:10.1186/1471-2148-11-334

Published: 17 November 2011

Additional files

Additional file 1:

List of protein sequences obtained from BLASTP sequence similarity search for LmjMETA1 against non-redundant NCBI database. Table S1. Description of BLASTP hits with their e-value.

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Open Data

Additional file 2:

List of reference genes for CAI determination. Table S2. Description of reference genes used for CAI determination.

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Additional file 3:

Codon Adaptation Index (CAI) of META1 homologs in T. cruzi. Figure S1. CAI of 2 META1 homologs in T. cruzi compared with RP S8 and α-Tubulin.

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Open Data

Additional file 4:

Quantitation of signal in western blots in Figure 5. Figure S2. Images of western blots in Figure 5B, C and 5D were quantitated by ImageJ software.

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Additional file 5:

Movie of superposed structures of HslJ (magenta), MxiM (yellow) and META1 model (green). Figure S3.

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Additional file 6:

Overexpression of META1 in L. donovani. Figure S4. Western blots (S4A and S4B) of L. donovani virulent and attenuated META1 transfectants with META1, BiP and GFP antibodies. S4C represents effect of META1 overexpression on growth kinetics of virulent and attenuated L. donovani compared to their respective wild-types.

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Additional file 7:

Mutagenesis of META1. Figure S5. Sequence alignment of META1 homologs and stereoscopic representation of META1 model highlighting the leucine 58 and leucine 80.

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Open Data

Additional file 8:

Effect of META1 overexpression in L. major. Figure S6. Effect of WT and mutant META1 overexpression in L. major on extracellular SAP activity (S6A) and growth kinetics (S6B). S6C and S6D represents western blot and QRT-PCR of L. major META1 transfectants respectively.

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Additional file 9:

Western blot of L. donovani whole cell lysates v/s culture supernatants. Figure S7. Western blot with GP63 and BiP on whole cell lysates and culture supernatants of L. donovani META1 transfectants.

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Additional file 10:

Phylogenetic tree re-rooted at two different roots. Figure S8. Phylogenetic tree representation generated in Figure 3 was re-rooted at Tolumonas (S8A) and T. cruzi Hypoth3 (S8B).

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Additional file 11:

List of protein sequences used in phylogenetic analysis. Table S3. Accession numbers of protein sequences.

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Additional file 12:

Primers used in the study. Table S4. List of sequences of all the primers used in this study.

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