Figure 4.

Histological analysis of Dlx gene expression patterns during caudal primary scale bud development. Transversal sections of whole mount hybridized tails from embryos at stages 29 to 31 illustrating the expression patterns of Dlx3 (A-D), Dlx4 (E-H), Dlx5 (I-L), Dlx1 (M-P) and Dlx2 (Q-T) during the development of the scales. Embryos are at stages 29 (B, E, F, I, M, N and Q), 30 (A, C, G, H, L, O, R-T) and 31 (D, K and P). EM, LM, ED and LD legends are as in Figure 2. For the LM stage, we illustrate the late LM stage when Dlx genes are predominantly expressed in the mesenchyme of scale buds. Dotted lines indicate the basal lamina separating the dental epithelium and the underneath mesenchyme. Scale bars: 25 μm.