Open Access Research article

Hybridization of mouse lemurs: different patterns under different ecological conditions

Andreas Hapke1*, Mark Gligor12, S Jacques Rakotondranary3, David Rosenkranz1 and Oliver Zupke14

Author Affiliations

1 Institut für Anthropologie, Johannes-Gutenberg-Universität Mainz, Colonel-Kleinmann-Weg 2, 55099 Mainz, Germany

2 Forschungszentrum Jülich GmbH, Projektträger Jülich, BIO1, 52425 Jülich, Germany

3 Department of Animal Ecology and Conservation, University of Hamburg, Biozentrum Grindel, Martin-Luther-King Platz 3, 20146 Hamburg, Germany

4 III. Medizinische Klinik und Poliklinik, Johannes-Gutenberg-Universität Mainz, Langenbeckstr. 1, 55131 Mainz, Germany

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BMC Evolutionary Biology 2011, 11:297  doi:10.1186/1471-2148-11-297

Published: 11 October 2011

Additional files

Additional file 1:

Individual trap positions at Mangatsiaka and Tsimelahy. The table contains geographical coordinates of the individual trap positions at Mangatsiaka and Tsimelahy, which we used for analyses of spatial overlap.

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Additional file 2:

Genotypes. The table contains the multi-locus genotypes of all individuals. Columns C and D contain the mitochondrial haplotypes and GenBank accession numbers. Column E lists the species-assignment as revealed by tree reconstructions based on mitochondrial haplotypes. Columns F to N contain the microsatellite genotypes in Genepop two-digit format.

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Additional file 3:

Proportion of Microcebus murinus within the nearest neighborhood of M. griseorufus. The figure displays the average proportion of individuals with murinus-like mitochondrial haplotypes within different numbers of nearest neighbors to individuals with griseorufus-like haplotypes. We used three datasets: Mtk: Mangatsiaka; Mtk_Riv: Mangatsiaka, all murinus-like individuals sampled more than 50 m from the nearest watercourse excluded; Tml: Tsimelahy. For each dataset, we calculated the proportion based on all individuals (_all) and as an average over 100 randomly resampled datasets, where we reduced the number of murinus-like individuals to the number of griseorufus-like individuals (_res).

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Additional file 4:

Diameters of neighborhoods with different numbers of nearest neighbors. The figure displays the average diameters of the nearest neighborhoods of individuals with griseorufus-like mitochondrial haplotypes at Mangatsiaka and Tsimelahy. We calculated average diameters for neighborhoods including different numbers of nearest neighbors based on three datasets: Mtk: Mangatsiaka; Mtk_Riv: Mangatsiaka, all murinus-like individuals sampled more than 50 m from the nearest watercourse excluded; Tml: Tsimelahy. For each dataset, we calculated diameters based on all individuals (_all) and as an average over 100 randomly resampled datasets, where we reduced the number of murinus-like individuals to the number of griseorufus-like individuals (_res).

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Additional file 5:

Simulation A, STRUCTURE, observed proportions with a threshold of 0.1. The table displays the results of simulation A with STRUCTURE with a threshold value for hybrid detection of 0.1.

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Additional file 6:

Simulation B: scenario of no hybrids at Mangatsiaka. The table displays the results of simulation B.

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Additional file 7:

Re-evaluated identification of hybrids in the gradient contact zone. The figure displays some of the original results of Gligor et al. [30] from the gradient contact zone, which we re-evaluated under application of the same criteria for hybrid detection as in the study presented here. Upper row: mitochondrial data (mt), middle and lower row membership coefficients observed with STRUCTURE with K = 2 in a large and a smaller dataset. Each vertical bar represents one individual. Colors represent the two species. Mv, Be, Amp, Sak, Ank, Anj, Pe, Man, Lok: abbreviations of sampling sites. Sampling sites are aligned in west-eastern direction along the transect sampled by Gligor et al. [30]. Gligor et al. [30] present the exact localities and full names of these sites in their Figure 1 and Table 1.

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