FISH mapping of the genes on polytene chromosome of A. gambiae, A. arabiensis and A. merus. FISH was performed with a probe specific to AgAcp34A-1 and a probe common to AgAcp34A-2 and AgAcp34A-3 on polytene chromosomes of A. gambiae (top panel), A. arabiensis (middle panel), and A. merus (bottom panel). Overlapping red and blue signals on chromosomes counterstained with YOYO-1 are shown on merged images (a, d, g). The AgAcp34A-2/3 probe is labeled with Cy3-AP3-dUTP (red signal in b, e, h). The AgAcp34A-1 probe is labeled with Cy5-AP3-dUTP (blue signal in c, f, i). Arrows indicate the site of localizations of the blue and red signals in subdivision 34A of the 3R arm.
Mancini et al. BMC Evolutionary Biology 2011 11:292 doi:10.1186/1471-2148-11-292