Open Access Research article

Molecular characterization and evolution of a gene family encoding male-specific reproductive proteins in the African malaria vector Anopheles gambiae

Emiliano Mancini1*, Francesco Baldini2, Federica Tammaro1, Maria Calzetta1, Aurelio Serrao2, Phillip George3, Isabelle Morlais4, Daniel Masiga5, Igor V Sharakhov3, David W Rogers6, Flaminia Catteruccia27 and Alessandra della Torre1

Author Affiliations

1 Istituto-Pasteur - Fondazione Cenci Bolognetti, Dipartimento di Sanità Pubblica e Malattie Infettive, Sapienza Università di Roma, Rome, Italy

2 Dipartimento di Medicina Sperimentale e Scienze Biochimiche, Università di Perugia, Terni, Italy

3 Department of Entomology, Virginia Tech, Blacksburg, VA, USA

4 Laboratoire d'entomologie médicale, OCEAC-IRD, BP288, Yaoundé, Cameroon

5 Molecular Biology and Bioinformatics Unit, International Centre of Insect Physiology and Ecology, Nairobi, Kenya

6 Max-Planck Institute for Evolutionary Biology, Plön, Germany

7 Division of Cell and Molecular Biology, Imperial College London, London, UK

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BMC Evolutionary Biology 2011, 11:292  doi:10.1186/1471-2148-11-292

Published: 6 October 2011

Additional files

Additional file 1:

Detection of paralog-specific transcripts in female and male tissues of A. gambiae, A. arabiensis and A. merus. Gene-specific nested RT-PCR using cDNA obtained after RNA extraction from the whole body of females (F-WB), male carcasses (M-C) and male accessory glands (M-MAG) as templates. For each tissue, A. gambiae products were run in the first lane, A. arabiensis products in the second lane and A. merus products in the third lane. Genomic DNA (gDNA) was amplified simultaneously to check for the efficiency of nested PCR reactions (e.g., primer annealing efficiency) in all analysed species. Ribosomal protein rpS7 was used to exclude genomic DNA contamination of cDNA templates (expected product size: cDNA = 458 bp, gDNA = 610 bp).

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