Additional file 2.

Figure S2. Multiple sequence alignment of mutY-amplicons from Calyptogena clam symbiont genomes. The mutY-containing genome region (mutY-amplicon) was amplified with a primer set [mutY_F and mutY_R (Table 2)] from 9 Calyptogena clam symbionts in PCR and from 2 symbiont genomes [Vesicomyosocius okutanii (Vok: accession # = AP009247) and Ruthia magnifica (Rma: accession # = CP000488)] in in silico PCR. Abbreviations of symbionts are shown in Table 3. Gray background horizontal sequence in Cpha S, part of metA (homoserine O-succinyltransferase); blue background sequence in Cpha S, mutY. *Identical nucleotide in the aligned sequences. Gray vertical column, gap in Cpha S sequence. Red letters, in-frame start codon of the original or mutated mutY ORFs. Blue letters, in-frame stop codon.

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Kuwahara et al. BMC Evolutionary Biology 2011 11:285   doi:10.1186/1471-2148-11-285