Open Access Research article

Sex in the PAC: A hidden affair in dark septate endophytes?

Pascal L Zaffarano1, Valentin Queloz1, Angelo Duò1 and Christoph R Grünig12*

Author Affiliations

1 Institute of Integrative Biology (IBZ), Forest Pathology and Dendrology, ETH Zurich, 8092 Zürich, Switzerland

2 Microsynth AG, Schützenstrasse 15, 9436 Balgach, Switzerland

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BMC Evolutionary Biology 2011, 11:282  doi:10.1186/1471-2148-11-282

Published: 30 September 2011

Additional files

Additional file 1:

Testing the presence of both idiomorphs in selected PAC strains. Testing the presence of both idiomorphs, or the absence of MAT genes in selected PAC strains.

Format: XLS Size: 23KB Download file

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Additional file 2:

Spatial distribution of mating types. Example of the spatial distribution of mating types for a population of Phialocephala subalpina and Phialocephala turicensis.

Format: EPS Size: 345KB Download file

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Additional file 3:

Sites in MAT genes identified to evolve under positive selection. Sites under positive selection were identified using the Bayes Empirical Bayes analysis under different CODML site models in the PAML software package.

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Additional file 4:

Phylogenies inferred from MAT genes. Phylogenies inferred for the three genes MAT1-1-1, MAT1-1-3, and MAT1-2-1. The sequence of Acephala applanata served as outgroup in all three analysis.

Format: EPS Size: 388KB Download file

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Additional file 5:

Sequencing strategy for MAT idiomorphs. Sequencing strategy applied to sequence the complete MAT idiomorphs for PAC species. Sequences of primers are given in the Additional file 5.

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Additional file 6:

Sequencing primers. Sequences of primers used to sequence the complete MAT idiomoprh of PAC species.

Format: XLS Size: 18KB Download file

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