Table 5

Procrustes ANOVA for the coral example, with a symmetry group consisting of reflection and rotation of order 2

Source

Degrees of freedom

Sums of squares

Mean squares

F

P

Individual

1127

4.9380

0.0043815

1.82

< 0.000001

Rotation

23

0.096360

0.0041896

4.20

< 0.000001

Reflection

23

0.16058

0.0069818

1.34

0.13

Rotation × reflection

23

0.072451

0.0031501

3.17

< 0.000001

Rotation × individual

1127

1.1251

0.0009983

4.95

< 0.000001

Reflection × individual

1127

5.8839

0.0052209

25.87

< 0.000001

Rotation × reflection × individual

1127

1.1184

0.0009924

4.92

< 0.000001

[Total FA]

3381

8.1274

0.0024039

Imaging error

4600

0.92842

0.0002018

1.26

< 0.000001

Digitizing error

9200

1.4789

0.0001607

The main effect of individuals is tested against the mean square for the total fluctuating asymmetry ("Total FA": pooling sums of squares and degrees of freedom across all three subspaces with asymmetric variation: rotation × individual, reflection × individual and rotation × reflection × individual). This total asymmetry is not used otherwise in the analysis.

Savriama and Klingenberg BMC Evolutionary Biology 2011 11:280   doi:10.1186/1471-2148-11-280