Luciferase assay of the CR1 and CR3 regions. Transactivation activity of Smad3, Stat3 and Klf4 on the conserved regions (CR1 and CR3) was investigated by the dual-glow luciferase assay. Smad3 shows elevated transactivation activity on CR3. Stat3 leads to transcriptional repression on CR1. Klf4 binding results in a repressive effect on CR1 and CR3.
Struckmann et al. BMC Evolutionary Biology 2011 11:267 doi:10.1186/1471-2148-11-267