Evaluation of triacylglyceride biosynthesis in yeast mutants complemented with castor bean DGAT1 or DGT2. The H1122 strain is an are1 are2 double mutant, while H1246 is an are1 are2 Iro1 dga1 quadruple mutant. Lipids were separated in a silica-gel plate of thin-layer chromatography, and the separation range corresponding to Triacylglicerol (TAG) and ADAG (mono-alkyl-diacylglicerol), used as lipid standards, is shown after Coomassie-Blue R250 staining.
Turchetto-Zolet et al. BMC Evolutionary Biology 2011 11:263 doi:10.1186/1471-2148-11-263