Predicted transmembrane domain for plants A. thaliana (Ath), B. napus (Bna), G. max (Gma), M. truncatula (Mtr), R. comunis (Rco), P. trichocarpa (Ptr), O. sativa (Osa), and Z. mays (Zma) DGAT1 and DGAT2 sequences. See Additional File 1 for sources. The TMHMM web tools of the Center for Biological Sequence Analysis, Technical University of Denmark TMHMM Server plots the probability of the ALDH sequence forming a transmembrane helix (0-1.0 on the y-axis) (shown in red for the relevant amino acid sequences) http://www.cbs.dtu.dk/services/TMHMM/ webcite. Eight and nine predicted transmembrane helices are identified for DGAT1 sequences, while two to four transmembrane helix (or helices) were observed for DGAT2 sequences; regions of all sequences predicted to be located inside or outside the membrane are shown in blue and pink, respectively. The Pfam:Mboat is represented by a dotted line to DGAT1, ACAT1 and ACAT2.
Turchetto-Zolet et al. BMC Evolutionary Biology 2011 11:263 doi:10.1186/1471-2148-11-263