Quantitative gene expression analysis of Acyrthosiphon pisum slimfast paralogs. Gene expression profiles are shown for three A. pisum lineages (LSR1, 7A, 9-2-1). Transcript levels for each slimfast paralog were quantified by QPCR on cDNA for whole adult asexual females at long day (AFL) and short day (AFS), sexual females (SF), and males (M). The relative abundance of each paralog was normalized to the housekeeping gene GAPDH (ACYPI009769) and expression levels were standardized by converting them to z-scores and compiled into a heat map (for details, see methods). Expression was not quantified for ACYPIslif1-3 because sequence similarity and AT richness in the untranslated regions precluded our ability to design paralog-specific QPCR primers. Aphid line 9-2-1 does not produce asexual females at short day so we did not measure AFS expression for 9-2-1 (see methods for details). Yellow: z > 0; Blue: z < 0.
Duncan et al. BMC Evolutionary Biology 2011 11:253 doi:10.1186/1471-2148-11-253