Email updates

Keep up to date with the latest news and content from BMC Evolutionary Biology and BioMed Central.

Open Access Highly Accessed Research article

Fossil gaps inferred from phylogenies alter the apparent nature of diversification in dragonflies and their relatives

Robert B Davis12*, David B Nicholson134, Emily LR Saunders1 and Peter J Mayhew1

Author Affiliations

1 Department of Biology, University of York, York, YO10 5YW, UK

2 Department of Zoology, University of Tartu, Vanemuise 46, EE-51014 Tartu, Estonia

3 Department of Palaeontology, The Natural History Museum, Cromwell Road, London, SW7 5BD, UK

4 National Museums of Scotland, Department of Natural Sciences, Edinburgh, Midlothian, EH1 1JF, UK

For all author emails, please log on.

BMC Evolutionary Biology 2011, 11:252  doi:10.1186/1471-2148-11-252


The electronic version of this article is the complete one and can be found online at: http://www.biomedcentral.com/1471-2148/11/252


Received:8 April 2011
Accepted:14 September 2011
Published:14 September 2011

© 2011 Davis et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background

The fossil record has suggested that clade growth may differ in marine and terrestrial taxa, supporting equilibrial models in the former and expansionist models in the latter. However, incomplete sampling may bias findings based on fossil data alone. To attempt to correct for such bias, we assemble phylogenetic supertrees on one of the oldest clades of insects, the Odonatoidea (dragonflies, damselflies and their extinct relatives), using MRP and MRC. We use the trees to determine when, and in what clades, changes in taxonomic richness have occurred. We then test whether equilibrial or expansionist models are supported by fossil data alone, and whether findings differ when phylogenetic information is used to infer gaps in the fossil record.

Results

There is broad agreement in family-level relationships between both supertrees, though with some uncertainty along the backbone of the tree regarding dragonflies (Anisoptera). "Anisozygoptera" are shown to be paraphyletic when fossil information is taken into account. In both trees, decreases in net diversification are associated with species-poor extant families (Neopetaliidae, Hemiphlebiidae), and an upshift is associated with Calopterygidae + Polythoridae. When ghost ranges are inferred from the fossil record, many families are shown to have much earlier origination dates. In a phylogenetic context, the number of family-level lineages is shown to be up to twice as high as the fossil record alone suggests through the Cretaceous and Cenozoic, and a logistic increase in richness is detected in contrast to an exponential increase indicated by fossils alone.

Conclusions

Our analysis supports the notion that taxa, which appear to have diversified exponentially using fossil data, may in fact have diversified more logistically. This in turn suggests that one of the major apparent differences between the marine and terrestrial fossil record may simply be an artifact of incomplete sampling. Our results also support previous notions that adult colouration plays an important role in odonate radiation, and that Anisozygoptera should be grouped in a single inclusive taxon with Anisoptera, separate from Zygoptera.

Background

Understanding past changes in biodiversity is a fundamental part of predicting the future of the Earth's ecosystems [1,2]. Phylogenies and the fossil record provide two complementary windows on temporal variation in biodiversity. The most traditional, palaeontological approach is to document the gain and loss of taxa in the fossil record without recourse to phylogenetic information [3-6]. Although the fossil record provides a direct timescale for observations and contains explicit information about extinction, it is incomplete, and more robust at higher taxonomic levels. A more recent, neontological approach is to analyse the shape of phylogenetic trees of extant taxa, revealing heterogeneity in the net rate of cladogenesis across taxa or through time [7,8]. This allows researchers to compare like with like through identifying sister taxa, but relies on the fossil record to provide a direct timescale, and does not contain explicit information about extinction. Combining fossil with phylogenetic information provides the potential therefore to combine the advantages of both to make more robust inferences about macroevolution [9,10]. In this paper we first summarise phylogenetic information for a long-lived clade of insects, the Odonatoidea (dragonflies and their relatives) and then combine it with fossil information to infer temporal changes in their diversification.

Identifying changes in the rate of diversification is central to understanding macroevolutionary processes. The simplest model of clade growth is an exponential one, where the rate of increase of taxa is constant through time [11,12]. The next simplest alternative is logistic growth, an equilibrium model with the growth of the clade declining as richness rises, through competition. Establishing whether or not clade growth follows expansionist or equilibrial models can therefore contribute towards establishing whether biotic interactions are important in macroevolution, as implied by the Red Queen paradigm [1,13]. Rates of speciation and extinction are also commonly variable across clades [7]. Identifying which evolutionary branches have experienced shifts in their macroevolutionary rates is therefore a first step towards establishing which evolutionary or ecological events might be responsible.

The insects comprise the majority of extant described species. Previous work on insect macroevolution [14] began with the traditional taxic approach from palaeontology [4,15], to which was added the neontological phylogenetic approach [16-19] which has identified a number of evolutionary and ecological processes that have shaped insect diversity [14]. Data have suggested that overall rise in taxonomic richness may be declining modestly towards the present [4], although not strongly so [15], and not in many recent radiations [14]. This is consistent with the generally exponential increase in taxa in the terrestrial fossil record [5,11,12,15] in comparison to the marine record [6]. However the relative incompleteness of the insect record may provide a source of bias, because the true originations of clades likely occurred prior to their first appearance in the fossil record. Using phylogenies to infer ghost ranges, Davis et al. [19] showed that a number of insect orders likely originated prior to their first fossil appearance, making the increase in orders through time look more logistic. The analysis of order-level trends however raises problems; there is a greater risk of paraphyly, complicating the estimation of ghost ranges, and changes in order richness do not necessarily reflect changes at lower taxonomic levels [20]. It would therefore be useful to examine patterns of diversification at a lower taxonomic level, in a clade of insects of equivalent age to the whole class, which previous studies have only achieved using the traditional taxic palaeontological approach.

Despite being less speciose than many other insect orders, the Odonatoidea (Odonata plus the extinct Protodonata) (= Holodonata [21], = Neodonatoptera [22]) are among the most ancient of all living continental fauna, with a fossil record extending back 320 million years, surviving several mass-extinction events [21]. Their accessibility as study systems has informed on many questions in ecology, evolution, and conservation biology [23]. This study aims to summarise existing phylogenetic information on the Odonatoidea by constructing a supertree at the family level. We first apply a neontological approach to the tree to detect where shifts in the net rate of diversification have occurred, and then combine the phylogenetic information with fossil record data to observe patterns of family-lineage richness over time.

Methods

Taxonomy

While previous family-level supertree studies of insects [18,24] have used the taxonomic nomenclature of Gordh & Headrick [25] for extant families and Ross & Jarzembowski [26] and the EDNA fossil insect database [http://edna.palass-hosting.org webcite] for extinct families studies, it is evident from examining more recent literature that views of odonate taxonomy have since altered, particularly with respect to fossil families [21]. Using the above references as our starting point, we thoroughly scoured recent literature to build up a taxonomy which represents more recent views, including new families and addressing synonymy. This is provided with references in additional file 1. Any synonyms used in input trees were corrected accordingly to ensure that only one name was used per taxon, preventing the same taxon appearing in the tree in two different positions. The family Eugeropteridae represents the separate order Geroptera and has been used as the supertree outgroup, in line with previous work [19,21,27].

Additional file 1. Valid taxonomy. A list of Odonata and Protodonata family names which are considered valid for the purposes of this study.

Format: PDF Size: 93KB Download file

This file can be viewed with: Adobe Acrobat ReaderOpen Data

Input trees

Papers containing Odonata phylogenies were searched for online using Google Scholar, Web of Knowledge and Science Direct databases. References cited in the studies found were used to find additional papers. As input tree searches were carried out until February 2010, no papers published after that time are here considered. Only papers post-1969 were used, being published after the groundbreaking study by Hennig [28] on insect classification and cladistic methodology.

Family-level Odonata phylogenies, constructed from either molecular or morphological data, were collected. In genus- and species-level phylogenies, taxa from the same family were reduced into a single leaf. In the case of a valid family (using our taxonomy) emerging as paraphyletic in a species level tree, all lineages were condensed into a single branch as this has no bearing on relationships with other families, and any valid families that were shown as polyphyletic were removed on grounds of uncertain placement following Davis et al. [18]. Trees where families were grouped together as higher taxa (e.g. into superfamilies) were not used. In total, 32 phylogenies from 23 papers were collected (see additional file 2).

Additional file 2. Input trees. Input trees from primary literature used in supertree analysis.

Format: PDF Size: 67KB Download file

This file can be viewed with: Adobe Acrobat ReaderOpen Data

Supertree methods

Two matrix-based methods were initially used for supertree construction: Standard Matrix Representation with Parsimony (MRP [29,30]), and Matrix Representation with Compatibility (MRC [31]). These methods have been shown to perform well compared to other methods in empirical studies [19] and cope with data sets containing high amounts of conflict [24]. MRP has been shown to perform well in simulation [32], and MRC has been shown to perform similarly well [33]. For each method, a majority rule tree with minority components was constructed from all equally optimal trees returned. Where there is less than a 50% majority for a relationship between particular taxa in equally optimal trees, the relationship appearing more times than any other is taken in the consensus. Fully bifurcating trees were required for diversification analyses (see below). For software and settings see additional file 3. Distance supertree methods were not considered as these have performed poorly compared to matrix-based methods in similar empirical studies [18,19,24]. Methods such as matrix representation with flipping (MRF) and quartet fit (QFIT) were not possible to use as our dataset proved too computationally demanding to run using available software [34,35] without compartmentalising the data set multiple times. Furthermore, we also avoid using strict supertree methods [see 36] which identify only relationships common to all input trees. There is unavoidably conflict between relationships in input trees collected from the literature and the aim of our work is to find an optimum solution rather than only summarise the areas of greatest certainty regarding phylogenetic relationships.

Additional file 3. Software & settings. Software used and settings applied for both MRP and MRC supertree analysis.

Format: PDF Size: 68KB Download file

This file can be viewed with: Adobe Acrobat ReaderOpen Data

The dataset was then refined to account for data non-independence, following the protocol outlined in Davis et al. [24]. Thirty input trees were used in the final supertree analyses. The refined dataset was then used to construct phylogenies using both the standard MRP and MRC methods.

We use the V index [37] to measure support as used in previous supertree studies [18,19,24,38]. This considers the number of input trees in agreement and in conflict with relationships in the supertree on a scale running from -1 (all conflicting) to +1 (all supporting). An input tree and supertree are in agreement when the relationship in the input tree directly matches that in the supertree (accounting for missing taxa in input trees). Conversely, where there is direct disagreement in a relationship between supertree and input tree, this is conflict. Input trees not relevant to the supertree node in question are not considered. For each supertree node, a score is given (either -1 or +1) for each input tree in the data set. For example, in a supertree study with 10 input trees, the supertree node would receive 10 individual scores which are averaged to give the overall nodal support value. The average score of all nodes gives a total V score for each supertree. Rather than V scores alone, V+ scores were also considered, because as well as relationships found directly in the dataset, they also consider as supporting relationships those that could be permitted by the dataset and do not directly conflict (e.g. polytomies). For a detailed discussion of the V index and supertree support see Wilkinson et al. [37].

Diversification analysis and taxon age

Significant upshifts and downshifts in the rate of diversification throughout the MRP and MRC majority rule trees were detected using the Slowinski-Guyer measure of imbalance, which compares the species richness of all extant sister clades [39-41]. The number of species in each family was obtained from the World Odonata List [http://www.pugetsound.edu/academics/academic-resources/slater-museum/biodiversity-resources/dragonflies/world-odonata-list webcite]. Any differences between this list and our taxonomy were accounted for (e.g. the inclusion of families we recognise as valid grouped under another family name were separated). Species numbers are shown in Table 1. This measure uses the assumption that, although diversification rates were not constant, at any point in time the diversification between two clades occurred at the same rate. Under this null model, the probability of observing an equal or greater difference in species richness between sister groups is given by 2[Nsmall/(Nlarge+Nsmall-1)]. This calculation is carried out through all extant sister clades in the phylogeny. Assuming that sister groups originated at the same time, effects of the ages of clades on diversification between sister groups can be accounted for. However, with this approach, there is the possibility that significant shifts at nodes will have an effect on those nodes further down the tree, known as the 'trickle-down effect'. The method used by Davies et al. [41] of adjusting the species richness in significant clades was used to eliminate this effect [see [18,19,24,42]]. Alternative methods are available for detecting the location of shifts in diversification across a phylogeny, but are not suitable for our data. For example the SymmeTREE approach [43] employs likelihood-based shift statistics but requires a resolved or nearly-resolved phylogeny at species level. Medusa [44] can be applied to an incompletely-resolved phylogeny but requires a fully dated tree.

Table 1. Species richnesses of extant families

Family-level richness over time was also documented. Families which could not be included in the phylogeny, because phylogenetic information is unavailable, were however included when looking at family-level richness over time, to provide as complete a picture as possible. Using literature published up to the end of 2009, the origination dates of known families were taken from their first appearance in the fossil record and extinction dates of extinct taxa from their last appearance. Dates are in line with a recent geological timescale [45,46] (see additional file 4). Using this information, the number of family lineages present in each stage through geological time can be plotted. For a discussion on the use of the term "lineages" here, rather than families sensu stricto see Davis et al. [19]. This richness was then corrected according to information in both the MRP and MRC phylogenies, by adjusting family lineage origination dates based on ghost ranges. Assuming that sister taxon lineages must have both been present when the divergence between them occurred, and that differences between the fossil record and phylogenies were caused by an incomplete fossil record and not an incorrect phylogeny, sister lineage origination dates were altered to equal that of the earlier sister group. The effect of both the standard MRP and MRC phylogenies was examined.

Additional file 4. Origination & extinction dates. Fossil first appearance dates and extinction dates for all Odonata and Protodonata families, and modified origination dates when ghost ranges are taken into account.

Format: PDF Size: 122KB Download file

This file can be viewed with: Adobe Acrobat ReaderOpen Data

Tests for exponential or logistic increase in family-lineage richness

To conduct time series analysis it is generally preferable to have equally-spaced sampling intervals. This was achieved by generating from the original data, new interpolated time-series of the same length, but equally spaced, using akima splines [47], using the aspline function in the Akima package in R. The expectation from exponential growth is that the logarithm of number of taxa will increase linearly over time. Logistic growth, where the rate of increase in taxon richness declines at high richness, can therefore be inferred from significant non-linearity (i.e. a deceleration of log richness nearer the present), which can be established by comparing the fit of linear and quadratic regressions of log(richness +1) against time. We tested for temporal autocorrelation in the linear regression by the Durbin-Watson test. All residual series showed significant positive autocorrelation at lag 1, and in all cases examination of the autocorrelation and partial autocorrelation functions indicated that the residuals were well described by an autoregression model of order 1 (AR(1)). We therefore accounted for this by using generalized least squares to model the data with an AR(1) residual correlation structure [48], and compared the linear and quadratic models. Modeling was performed in R [49] using the gls function from the nlme library.

Results

Phylogeny

Both the MRP and the MRC phylogenies (see Figure 1) show the divergence of the Zygoptera (damselflies) and Anisoptera (true dragonflies) + "Anisozygoptera" into two robustly supported monophyletic groups (V scores: Zygoptera, +0.280; Anisoptera + "Anisozygoptera", +0.714). The Anisoptera are nested within a paraphyletic assemblage of "anisozygopteran" families, comprising five main lineages, including the extant family Epiophlebiidae belonging to its own exclusive lineage. The whole crown group of extant odonate families nests within a paraphyletic assemblage of fossil protodonate and odonate families. The backbone of the tree as a whole in both MRP and MRC analyses is robustly supported with primarily positive V scores for clades. The only clade receiving a negative V score is crown group Odonata (V = -0.111), though there is some uncertainty regarding the backbone in Anisoptera, where some clades are not found in all equally optimal trees and there are differences in the most commonly found relationships between MRP and MRC analyses. However, the MRP and MRC trees are largely similar at lower levels (Figure 1). For V and V+ scores of individual clades see additional file 5.

thumbnailFigure 1. Supertree phylogeny of Odonatoidea based on MRC and MRP analyses of 30 input trees. The strict consensus between MRC and MRP supertrees is supplemented where MRC and MRP topologies differ. Taxa in grey marked with * are extinct. Taxa in black are extant.

Additional file 5. V and V+ scores. V and V+ support scores for MRP and MRC supertree nodes.

Format: PDF Size: 87KB Download file

This file can be viewed with: Adobe Acrobat ReaderOpen Data

Diversification

Both the MRP and MRC majority rule supertrees show significant shifts in net rates of diversification (Figure 1). Both show downshifts in the single family lineages Hemiphlebiidae (Zygoptera) and Neopetaliidae (Anisoptera), and a downshift in the lineage leading to Pseudolestidae + Dicteriadidae + Polythoridae + Calopterygidae (Zygoptera), before a subsequent positive shift in the lineage leading to Polythoridae + Calopterygidae. All shifts except that involving Neopetaliidae occur at the base of clades obtaining positive V scores, and in the case of Neopetaliidae, a significant downshift is detected despite differing placement in the two trees. Other upshifts and downshifts detected are shown in just one tree or the other. These shifts are detected in the region of uncertainty regarding the backbone of the Anisoptera clade - a downshift in the Petaluridae lineage (MRP), or a downshift in a clade comprising Epiophlebiidae + Petaluridae + Lindeniidae + Gomphidae + Austropetaliidae + Aeshnidae + Neopetaliidae + Cordulegastridae + Synthemistidae + Macromiidae + Libellulidae + Corduliidae, then a subsequent upshift in this clade to the exclusion of Epiophlebiidae and Petaluridae (MRC). See additional file 6 for sister group species richness comparisons in full.

Additional file 6. Sister group species richness comparisons. Sister group species richness comparisons for both MRP and MRC supertrees indicating where significant upshifts and downshifts in species richness have occurred.

Format: PDF Size: 869KB Download file

This file can be viewed with: Adobe Acrobat ReaderOpen Data

Family richness, originations and extinctions

Aside from family extinctions at the end of the Permian and Triassic, a lineage through time plot based on fossil information only (Figure 2) shows an increase in the number of family-level lineages leading up to the Jurassic-Cretaceous boundary. The increase in richness is interrupted by extinctions and origination events throughout the Cretaceous, including a significant extinction at the Lower-Upper Cretaceous boundary, before a sharp rise in richness to the present.

thumbnailFigure 2. A lineage through time plot showing family-level richness, origination and extinction events over a geological timescale based on fossil information either alone, or in combination with either MRC or MRP phylogenetic information. For the geological timescale, C = Carboniferous, P = Permian, Tr = Triassic, J = Jurassic, K = Cretaceous, Pg = Palaeogene, Ng = Neogene, Q = Quaternary. Different coloured bands represent lower, middle (if applicable) and upper stages of each geological period [45,46].

The inclusion of ghost range information shows a slightly different scenario. Both MRP and MRC-based analyses give similar pictures. As expected with ghost ranges inferred, earlier originations occur in the phylogeny-adjusted models, and richness is higher (see Figure 2). With ghost ranges inferred, between 46 (MRC) and 50 (MRP) families have their fossil ranges extended (see additional file 4 for details), with the most dramatic extension for Epiophlebiidae (0 to 196.5 my). Although the same extinction events are apparent, the rise in family-level lineages is slightly sharper in the Triassic and rises dramatically during the Jurassic. The ultimate impact of this is that richness calculated using fossil information alone is only 57-64% of that calculated using phylogenetic information by the end of the Jurassic. Patterns of origination and extinction are similar in all scenarios through the Cretaceous, and while higher richness is evident in the Cenozoic with ghost ranges inferred, the level of richness is stable, compared to the rapid rise in richness found based only on fossil data.

Generalized least squares, accounting for temporal autocorrelation in the residuals, supports the inclusion of a quadratic (i.e. curvilinear) temporal component to log richness for both the MRC supertree (t = -2.65, df = 43, p = 0.011) and the MRP supertree (t = -3.12, df = 43, p = 0.0032). However, for the fossil data alone inclusion of the quadratic term is not well-supported (including extant richness t = -1.49, df = 43, p = 0.144; not including extant richness t = -1.93, df = 42, p = 0.06).

Discussion

Odonata phylogeny

Two alternative supertree analyses, representing the largest family-level phylogenetic analysis of Odonatoidea to date, converge on a very similar topology, with overall positive support, indicating general agreement between input trees regarding many relationships within the tree (Figure 1). Therefore based on primary studies from which input trees have been acquired, we can summarise the following consensus regarding relationships within Odonatoidea, following supertree analysis. Importantly, Zygoptera are a monophyletic suborder, separate to Anisoptera (+ "Anisozygoptera"), which refutes previous hypotheses suggesting Zygoptera could be a paraphyletic grade towards Anisoptera [50,51]. "Anisozygoptera", although considered a modern suborder, and regarded as such in recent phylogenetic analyses [e.g. [52]] based on the single extant family Epiophlebiidae, should not be given official taxonomic status. As can be seen, with inclusion of fossil taxa, "Anisozygoptera" is a paraphyletic assemblage with Anisoptera nested within.

It is important that the backbone of the tree receives mostly positive V support, and provides a solid framework to analyse evolutionary questions at a high level in Odonatoidea, though there are some areas of the tree which do remain problematic. The negative support (representing much conflict in the constituent input trees) at the node representing crown Odonata is initially concerning. However, there is much agreement for monophyletic Zygoptera and Anisoptera + "Anisozygoptera" and the negative support for the crown group as a whole represents uncertainty relating to the placement of the fossil family Tarsophlebiidae (i.e. is it included in the crown group or not?). In both supertrees Tarsophlebiidae sits outside the crown group, yet it is represented both within [53,54] and outside [55] of the crown group in input trees, and Bybee et al. [56] show these alternative scenarios in different analyses. Input trees do not concur well over the position of this taxon, and though the debate rolls on, recent fossil evidence supports the more basal position for Tarsophlebiidae that we find here [57].

The most important phylogenetic uncertainty to target in future work must be along the backbone of the Anisoptera clade with most topological differences between the two supertree methods occurring here, and this does affect the result of diversification analyses (see below). However, below the suborder level there is much agreement in relationships. The supertree presented here should be regarded as a work in progress since taxonomic revisions, discovery of new fossil forms, and new molecular data for the order are now commonly reported. Furthermore, the inclusion of more fossil families may help resolve the tree further, but many are yet to be included in phylogenetic analyses.

Significant shifts in diversification and potential morphological innovations

Previous work by Misof [58] identified two major characteristics within Anisoptera families that are positively correlated with species richness: the evolution of elaborate colour patterns and the evolution of sexual dimorphism in colouration (sexual dimorphism in body size is common in all Anisoptera [59]), features often linked to the process of sexual selection and its role in speciation.

Downshifts in net diversification are inferred from the low extant richness of Hemiphlebiidae, Neopetaliidae (both significant downshifts in both trees), Epiophlebiidae, Petaluridae (both significant downshifts in the MRP tree) and a clade containing Pseudolestidae (significant in both trees, preceding a later upshift in Polythoridae and Calopterygidae). All these families have simple body colouration patterns [60], consistent with Misof [58]. In addition the families Polythoridae and Calopterygidae both show dramatic bright and sexually dimorphic colouration and are associated with a robust diversification upshift in both trees. Our results therefore combine well with Misof's findings by demonstrating where identifiable radiations and restrictions have likely occurred. In addition to these features, Epiophlebiidae also has long larval stages (5-6 y), which could feasibly increase extinction risk or hinder speciation [14]. The Neopetaliidae has extremely specialised niches (riverside caves) possibly restricting its diversification. In the latter two cases though, such characteristics might just be those of surviving species rather than the families as a whole, so caution is required. A fuller determination of the causes of any shifts would benefit from an analysis at finer taxonomic resolutions. The use of simple topology-based statistics in the absence of fossil data and extinct taxa likely limits the inferences that can be made on diversification shifts, and may induce biases [see [61,62]]. Hence the findings presented here should be revisited as the opportunity to incorporate new data and more complex, and potentially realistic, diversification models presents itself.

Family richness through time

Fossil record-only analysis follows some similar short term variation to the phylogeny-based analyses, but phylogeny-based analyses show some noticeably higher increases in family-level lineage richness through the Triassic and Jurassic, and we show that richness was likely much higher through the Cretaceous and then consequently the Cenozoic than can be shown by using the fossil record alone (Figure 2). It is most probable that the extensions of fossil ranges we infer are quite conservative [63], but the impact of including such information is evident in our analyses. The divergence between the fossil and phylogenetic richness curves (Figure 2) accumulates gradually throughout the timeline, and is notable towards the end of the Jurassic, suggesting that many lineages with much later first fossils are rather first found here. This may of course partly represent an abundance of suitable fossil-bearing strata instead of, or as well as, a genuine set of first originations. It therefore remains possible that the taxa involved actually have still earlier origination dates. If true however, this would only strengthen our findings by making the true richness curve appear even more logistic. Using fossil data alone there is a suggestion of a slow-down in the net rate of origination that is not significant, mirroring the insect fossil record as a whole [4]. Both the phylogenetic analyses however alter the fossil-based curve to a more logistic model, with a sudden increase in family richness through the Jurassic being the most noticeable difference. There has been debate in the past over whether the fossil record for all organisms exhibits logistic growth, or whether it has been exponential but punctuated with mass extinctions that limit the level of taxonomic diversity [64]. However, given that an approximately exponential increase in richness can turn to a logistic one after accounting for gaps in the fossil record, our results suggest that caution should be applied to other such cases, particularly in the terrestrial record where the evidence for logistic growth has so far been thinner [11,12,15].

After accounting for the general trend in taxonomic richness, variation around this trend remains. Some of this coincides with well-documented mass-extinctions and recoveries, including here the end-Permian, but in other cases, such as the end-Cretaceous, no effect is seen [see also 65]. In the fossil record as a whole, fluctuations are known to correlate with a number of environmental and geophysical variables [66,67]. The sudden increase in richness in the Jurassic coincides with an icehouse climate mode [68], as is the case for the initial radiation of Odonatoidea in the Carboniferous, consistent with the findings of Mayhew et al. [66]. Also, notable changes to freshwater ecosystems occurred in the Jurassic with the radiation of aquatic Diptera and other animal taxa that are today important food sources for Odonata [69]. Though the environmental correlates of Odonata richness through time requires a fuller study, our results suggest that such studies could benefit from incorporation of phylogenetic information.

Conclusions

Phylogenetic information provides one way to adjust for the imperfections and biases inherent in analyses of fossil data. When such information is used to address family-level richness in an ancient clade of insects, different models of macroevolution are supported. The more logistic pattern of diversification we observe when phylogenetic information is included in such a study is in contrast to the exponential pattern of increases in odonate richness suggested by the fossil record alone, and the terrestrial record more generally. These results therefore imply that some of the major differences between the marine and terrestrial records are artifacts of incomplete sampling. Our supertrees provide a baseline for further macroevolutionary, comparative and phylogenetic studies of Odonata and Protodonata.

Authors' contributions

All authors contributed to manuscript writing and have read and approved the final manuscript. RBD and ELRS collected input trees and conducted supertree analyses. RBD and DBN drew up a valid taxonomy and DBN collected all the information regarding origination and extinctions of lineages. RBD conducted sister group species richness analyses and constructed the lineage through time plots. PJM tested for logistic/exponential clade growth and coordinated the study as a whole.

Acknowledgements

RBD was supported by a BBSRC studentship. RBD was also supported by targeted financing project SF0180122s08 and by the European Union through the European Regional Development Fund (Center of Excellence FIBIR).

References

  1. Alroy J: Dynamics of origination and extinction in the marine fossil record.

    PNAS 2008, 105(Supplement 1):11536-11542. PubMed Abstract | Publisher Full Text | PubMed Central Full Text OpenURL

  2. Mayhew PJ: Global climate and extinction: evidence from the fossil record. In Climate Change, Ecology and Systematics. Edited by Hodkinson TR, Jones MB, Waldren S, Parnell JAN. Cambridge: Systematics Association Special Series, Cambridge University Press; 2011:99-121. OpenURL

  3. Sepkoski JJ, Bambach RK, Raup DM, Valentine JW: Phanerozoic marine diversity and the fossil record.

    Nature 1981, 293:435-437. Publisher Full Text OpenURL

  4. Labandeira CC, Sepkoski JJ: Insect diversity in the fossil record.

    Science 1993, 261:310-315. PubMed Abstract | Publisher Full Text OpenURL

  5. Benton MJ: Diversification and extinction in the history of life.

    Science 1995, 268:52-58. PubMed Abstract | Publisher Full Text OpenURL

  6. Alroy J, Aberhan M, Bottjer DJ, Foote M, Fürsich FT, Harries PJ, Hendy AJW, Holland SM, Ivany LC, Kiessling W, Kosnik MA, Marshall CR, McGowan AJ, Miller AI, Oiszewski TD, Patzkowsky ME, Peters SE, Viller L, Wagner PJ, Bonuso N, Borkow PS, Brenneis B, Clapham ME, Fall LM, Ferguson CA, Hanson VL, Krug AZ, Layou KM, Leckey EH, Nürnberg S, et al.: Phanerozoic trends in the global diversity of marine invertebrates.

    Science 2008, 321:97-100. PubMed Abstract | Publisher Full Text OpenURL

  7. Mooers AO, Heard SB: Inferring evolutionary process from phylogenetic tree shape.

    Q Rev Biol 1997, 72:31-54. Publisher Full Text OpenURL

  8. Ricklefs RE: Estimating diversification rates from phylogenetic information.

    Trends Ecol Evol 2007, 22:601-610. PubMed Abstract | Publisher Full Text OpenURL

  9. Lloyd GT, Davis KE, Pisani D, Tarver JE, Ruta M, Sakamoto M, Hone DWE, Benton MJ: Dinosaurs and the Cretaceous Terrestrial Revolution.

    Proc R Soc B Biol Sci 2008, 275:2483-2490. Publisher Full Text OpenURL

  10. Quental TB, Marshall CR: Diversity dynamics: molecular phylogenies need the fossil record.

    Trends Ecol Evol 2010, 25:434-441. PubMed Abstract | Publisher Full Text OpenURL

  11. Benton MJ: Models for the diversification of life.

    Trends Ecol Evol 1997, 12:490-495. PubMed Abstract | Publisher Full Text OpenURL

  12. Kalmar A, Currie DJ: The completeness of the continental fossil record and its impact on patterns of diversification.

    Paleobiology 2010, 36:51-60. Publisher Full Text OpenURL

  13. Benton MJ: New take on the Red Queen.

    Nature 2010, 463:306-307. PubMed Abstract | Publisher Full Text OpenURL

  14. Mayhew PJ: Why are there so many insect species? Perspectives from fossils and phylogenies.

    Biol Rev Camb Philos Soc 2007, 82:425-454. PubMed Abstract | Publisher Full Text OpenURL

  15. Eble GJ: Decline in extinction rates and scale invariance in the fossil record.

    Paleobiology 1999, 25:434-439. OpenURL

  16. Mayhew PJ: Shifts in hexapod diversification and what Haldane could have said.

    Proc R Soc B Biol Sci 2002, 269:969-974. Publisher Full Text OpenURL

  17. Mayhew PJ: A tale of two analyses: estimating the consequences of shifts in hexapod diversification.

    Biol J Linn Soc 2003, 80:23-36. Publisher Full Text OpenURL

  18. Davis RB, Baldauf SL, Mayhew PJ: Eusociality and the success of the termites: insights from a supertree of dictyopteran families.

    J Evol Biol 2009, 22:1750-1761. PubMed Abstract | Publisher Full Text OpenURL

  19. Davis RB, Baldauf SL, Mayhew PJ: Many hexapod groups originated earlier and withstood extinction events better than previously realized: inferences from supertrees.

    Proc R Soc B Biol Sci 2010, 277:1597-1606. Publisher Full Text OpenURL

  20. Lane A, Benton MJ: Taxonomic level as a determinant of the shape of the Phanerozoic marine biodiversity curve.

    Am Nat 2003, 162:265-276. PubMed Abstract | Publisher Full Text OpenURL

  21. Grimaldi D, Engel MS: Evolution of the Insects. Cambridge: Cambridge University Press; 2005. OpenURL

  22. Bechly G: Morphologische Untersuchungen am Flügelgeäder der rezenten Libellen und deren Stammgruppenvertreter (Insecta; Pterygota; Odonata) unter besonderer Berücksichtigung der Phylogenetischen Systematik und der Gundplanes der Odonata.

    Petalura 1996, 2:1-402. OpenURL

  23. Córdoba-Aguilar AE: Dragonflies and Damselflies: Model Organisms for Ecological and Evolutionary Research. Oxford: Oxford University Press; 2008. OpenURL

  24. Davis RB, Baldauf SL, Mayhew PJ: The origins of species richness in the Hymenoptera: insights from a family-level supertree.

    BMC Evol Biol 2010, 10:109. PubMed Abstract | BioMed Central Full Text | PubMed Central Full Text OpenURL

  25. Gordh G, Headrick DH: A Dictionary of Entomology. Wallingford: CABI Publishers; 2000. OpenURL

  26. Ross AJ, Jarzembowski EA: Arthropoda (Hexapoda: Insecta). In The Fossil Record 2. Edited by Benton MJ. London: Chapman & Hall; 1993:363-426. OpenURL

  27. Bechly G, Brauckmann C, Zessin W, Groning E: New results concerning the morphology of the most ancient dragonflies (Insecta: Odonatoptera) from the Namurian of Hagen-Vorhalle (Germany).

    J Zool Syst Evol Res 2001, 39:209-226. Publisher Full Text OpenURL

  28. Hennig W: Die Stammesgeschichte der Insekten. Frankfurt am Main: Waldemar Kramer; 1969. OpenURL

  29. Baum BR: Combining trees as a way of combining data sets for phylogenetic inference, and the desirability of combining gene trees.

    Taxon 1992, 41:3-10. Publisher Full Text OpenURL

  30. Ragan MA: Phylogenetic inference based on matrix representation of trees.

    Mol Phylog and Evol 1992, 1:53-58. Publisher Full Text OpenURL

  31. Rodrigo AG: On combining cladograms.

    Taxon 1996, 45:267-274. Publisher Full Text OpenURL

  32. Bininda-Emonds ORP, Sanderson MJ: Assessment of the accuracy of matrix representation with parsimony analysis of supertree construction.

    Syst Biol 2001, 50:565-579. PubMed Abstract OpenURL

  33. Ross HA, Rodrigo AG: An assessment of matrix representation with compatibility in supertree construction. In Phylogenetic Supertrees: Combining Information to Reveal the Tree of Life. Edited by Bininda-Emonds ORP. Dordrecht: Kluwer Academic Publishers; 2004:35-63. OpenURL

  34. Creavey CJ, McInerney JO: Clann: investigating phylogenetic information through supertree analyses.

    Bioinformatics 2005, 21:390-392. PubMed Abstract | Publisher Full Text OpenURL

  35. Chen D, Eulenstein O, Fernández-Baca D, Burleigh JG: Improved heuristics for minimum-flip supertree construction.

    Evol Bioinfomat 2:347-356. OpenURL

  36. Bininda-Emonds ORP: New uses for old phylogenies. In Phylogenetic Supertrees: Combining Information to Reveal the Tree of Life. Edited by Bininda-Emonds ORP. Dordrecht: Kluwer Academic Publishers; 2004:3-14. OpenURL

  37. Wilkinson M, Pisani D, Cotton JA, Corfe I: Measuring support and finding unsupported relationships in supertrtees.

    Syst Biol 2005, 54:823-831. PubMed Abstract | Publisher Full Text OpenURL

  38. Ruta M, Pisani D, Lloyd GT, Benton MJ: A supertree of Temnospondyli: cladogenetic patterns in the most species-rich group of early tetrapods.

    Proc R Soc B Biol Sci 2007, 274:3087-3095. Publisher Full Text OpenURL

  39. Slowinski JB, Guyer C: Testing the stochasticity of patterns of organismal diversity: an improved null model.

    Am Nat 1989, 134:907-921. Publisher Full Text OpenURL

  40. Sanderson MJ, Donoghue MJ: Shifts in diversification rate with the origin of angiosperms.

    Science 1994, 264:1590-1593. PubMed Abstract | Publisher Full Text OpenURL

  41. Davies TJ, Barraclough TG, Chase MW, Soltis PS, Soltis DE, Savolainen : Darwins abominable mystery: insights from a supertree of the angiosperms.

    PNAS 2004, 101:1904-1909. PubMed Abstract | Publisher Full Text | PubMed Central Full Text OpenURL

  42. Hunt T, Bergsten J, Levkanicova Z, Papadopoulou A, John OS, Wild R, Hammond PM, Ahrens D, Balke M, Caterino MS, Gómez-Zurita J, Ribera I, Barraclough TG, Bocakova M, Bocak L, Vogler AP: A comprehensive phylogeny of beetles reveals the evolutionary origins of a superradiation.

    Science 2007, 318:1913-1916. PubMed Abstract | Publisher Full Text OpenURL

  43. Chan K, Moore BR: SYMMETREE: whole-tree analysis of differential diversification rates.

    Bioinformatics 2005, 21:1709-1710. PubMed Abstract | Publisher Full Text OpenURL

  44. Alfaro ME, Santini F, Brock C, Alamillo H, Dornburg A, Rabosky DL, Carnevale G, Harmon LJ: Nine exceptional radiations plus high turnover explain species diversity in jawed vertebrates.

    Proc R Soc B Biol Sci 2009, 106:13410-13414. OpenURL

  45. Gradstein FM, Ogg JG, Smith AG: A Geologic Time Scale 2004. Cambridge: Cambridge University Press; 2004. OpenURL

  46. Ogg JG, Ogg G, Gradstein FM: The Concise Geologic Time Scale. Cambridge: Cambridge University Press; 2008. OpenURL

  47. Akima H: A New Method of Interpolation and Smooth Curve Fitting Based on Local Procedures.

    JACM 1970, 17:589-602. Publisher Full Text OpenURL

  48. Fox J: An R and S-PLUS companion to applied regression. Thousand Oaks, CA: Sage; 2002. OpenURL

  49. R Development Core Team:

    R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. 2005.

    ISBN 3-900051-07-0, URL http://www.R-project.org webcite

    OpenURL

  50. Saux C, Simon C, Spicer GS: Phylogeny of the Dragonfly and Damselfly Order Odonata as Inferred by Mitochondrial 12S Ribosomal RNA Sequences.

    Ann Entomol Soc Am 2003, 96:693-699. Publisher Full Text OpenURL

  51. Trueman J: A preliminary cladistic analysis of odonate wing venation.

    Odonatologica 25:59-72. OpenURL

  52. Dumont HJ, Vierstraete A, Vanfleteren JR: A molecular phylogeny of the Odonata (Insecta).

    Syst Entomol 2010, 35:6-18. Publisher Full Text OpenURL

  53. Fleck G, Bechly G, Martinez-Delclos X, Jarzembowski E, Coram R, Nel A: Phylogeny and classification of the Stenophlebioptera (Odonata: Epiproctophora).

    Ann Soc Entomol France 2003, 39:55-94. OpenURL

  54. Carle FL: The wing vein homologies and phylogeny of Odonata: A continuing debate.

    Soc Int Odonatol Rapid Comm 1982, 4:1-66. OpenURL

  55. Rehn AC: Phylogenetic analysis of higher-level relationships of Odonata.

    Syst Entomol 2003, 28:181-240. Publisher Full Text OpenURL

  56. Bybee SM, Ogden TH, Branham MA, Whiting MF: Molecules, morphology and fossils: a comprehensive approach to odonate phylogeny and the evolution of the odonate wing.

    Cladistics 2008, 24:477-514. Publisher Full Text OpenURL

  57. Huang D-Y, Nel A: The first Chinese Tarsophlebiidae from the Lower Cretaceous Yixian Formation, with morphological and phylogenetic implications (Odonatoptera: Panodonata).

    Cretac Res 2009, 30:429-433. Publisher Full Text OpenURL

  58. Misof B: Diversity of Anisoptera (Odonata): infering speciation processes from patterns of morphological diversity.

    Zoology 2002, 105:355-65. PubMed Abstract | Publisher Full Text OpenURL

  59. Corbet PS: Dragonflies: behavior and Ecology of Odonata. Ithaca: Cornell University Press; 1999. OpenURL

  60. Silsby J: Dragonflies of the World. Washington DC: Smithsonian Institution Press; 2001. OpenURL

  61. McInnes L, Orme CDL, Purvis A: Detecting shifts in diversity limits from molecular phylogenies: what can we know?

    Proc R Soc B Biol Sci, in press. OpenURL

  62. Tarver JE, Donoghue PCJ: The trouble with topology: phylogenies without fossils provide a revisionist perspective of evolutionary history in topological analyses of diversity.

    Syst Biol, in press. OpenURL

  63. Signor PW, Lipps J: Sampling bias, gradual extinction patterns and catastrophes in the fossil record.

    Geol Soc Am Spec Pap 1982, 190:291-296. OpenURL

  64. Purvis A, Hector A: Getting the measure of biodiversity.

    Nature 2000, 405:212-219. PubMed Abstract | Publisher Full Text OpenURL

  65. Labandeira CC: The fossil record of insect extinction: new approaches and future directions.

    Am Entomol 2005, 51:14-29. OpenURL

  66. Mayhew PJ, Jenkins GB, Benton TG: A long-term association between global temperature and biodiversity, origination and extinction in the fossil record.

    Proc R Soc B Biol Sci 2008, 275:47-53. Publisher Full Text OpenURL

  67. Cárdenas AL, Harries PJ: Effect of nutrient availability on marine origination rates throughout the Phanerozoic eon.

    Nature Geosci 2010, 3:430-434. Publisher Full Text OpenURL

  68. Frakes LA, Francis JE, Syktus JI: Climate Modes of the Phanerozoic. Cambridge: Cambridge University Press; 1992. OpenURL

  69. Wootton RJ: The historical ecology of aquatic insects: An overview.

    Palaeogeogr, Palaeoclimateol, Palaeoecol 1988, 62:477-492. Publisher Full Text OpenURL