Additional file 3.

Pheromone precursors of different red yeast species. (a) The organization of the genomic region encompassing the MAT A1 pheromone precursor genes in Sporobolomyces sp. IAM 13481 is shown on top. Coding regions of the pheromone precursor genes (RHA1, RHA2 and RHA3) are depicted by gray arrows, indicating the direction of transcription. (b) Alignment of the Rha2 pheromone precursor of different MAT A1 red yeast species/strains (Ss, Sporidiobolus salmonicolor; Sj, Sporidiobolus johnsonii; Sp, Sporobolomyces sp. IAM 13481; Rt, Rhodosporidium toruloides). Amino acids differing from the S. salmonicolor strain CBS 483 are shown in red. Sequence repeats proposed to represent the peptide moiety of the mature pheromone are shadowed and those resembling the CAAX motif are underlined. (c) Phylogenetic tree showing the relationships between RHA2 genes from the indicated red yeast species, based on the alignment of their coding sequences. Groups are the same as in (b). Sequences of strains depicted in boldface are shown in (b). The tree was inferred using Maximum Parsimony. Bootstrap values from 1000 replicates are shown in the tree nodes.

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Coelho et al. BMC Evolutionary Biology 2011 11:249   doi:10.1186/1471-2148-11-249