Table 1

Interaction of LNX1 and LNX2 with known MUPP1 ligands

Prey

Empty

Vector

MUPP1

(PDZ 10-13)

LNX1

(NPAY-PDZ4)

LNX1

(RING-PDZ2)

LNX2

ERBB2IP

(PDZ)

Nos1

(PDZ)

Dlg2









Bait

His3

β-gal

His3

β-gal

His3

β-gal

His3

β-gal

His3

β-gal

His3

β-gal

His3

β-gal

His3

β-gal


TAPP1

-

-

+++

+++

-

-

+++

+++

-

-

-

-

-

-

-

-

SynGAP1

-

-

+++

+++

++

-

+++

+++

+++

+++

-

-

-

-

+++

+++

EphA7

-

-

+++

++

+++

+

+++

+++

+++

+++

-

-

-

-

-

-

HTR2C

-

-

+++

+++

-

-

-

-

++

-

n/d

n/d

n/d

n/d

n/d

n/d


The ability of carboxyl terminal sequences from the indicated MUPP1 interacting proteins (baits) to interact with LNX1 and LNX2 (preys) was tested using the yeast two-hybrid system. An empty prey vector and a MUPP1 prey were included as negative and positive controls respectively. Interactions with unrelated PDZ domain-containing proteins (ERBB2IP, Nos1 and Dlg2) were assessed to demonstrate the specificity of binding to LNX proteins. Interactions between bait and prey were indicated by expression of two reporter genes - His3 and LacZ coding for β-galactosidase (β-gal). Truncated LNX1 constructs were used because the full-length protein was not functional in the yeast two-hybrid system. n/d = not determined.

Flynn et al. BMC Evolutionary Biology 2011 11:235   doi:10.1186/1471-2148-11-235

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