Open Access Highly Accessed Research article

Molecular evolution of a chordate specific family of G protein-coupled receptors

Stefan Kurtenbach1, Christoph Mayer2, Thomas Pelz3, Hanns Hatt1, Florian Leese4 and Eva M Neuhaus35*

Author Affiliations

1 Department of Cell Physiology, Ruhr University Bochum, Universitaetsstrasse 150, 44801 Bochum, Germany

2 Zoologisches Forschungsmuseum Alexander Koenig, Adenauerallee 160-163, 53113 Bonn, Germany

3 NeuroScience Research Center, Charité - Universitätsmedizin Berlin, Charitéplatz 1, 10117 Berlin, Germany

4 Department of Animal Ecology, Evolution and Biodiversity, Ruhr-University Bochum, Universitaetsstrasse 150, 44801 Bochum, Germany

5 author to whom correspondence should be addressed, Eva M. Neuhaus, NeuroScience Research Center, Charité, Charitéplatz 1, 10117 Berlin, Germany

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BMC Evolutionary Biology 2011, 11:234  doi:10.1186/1471-2148-11-234

Published: 9 August 2011

Additional files

Additional file 1:

Phylogenetic analysis. (A) Phylogenetic tree obtained by RAxML. (B) Screening of XRAIG4 expression in different tissues from Xenopus laevis by RT-PCR.

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Additional file 2:

Partial GPRC5 alignments. Alignment showing a sequence between the end of the N-terminus and the transition into transmembrane region 1 from different GPRC5 receptors.

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Additional file 3:

Analysis of the neighboring genomic regions surrounding of GPRC5 receptors. Genes in the vicinity of GPRC5 receptors from different species.

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Additional file 4:

Sequence logos. Sequence logos of all GPRC5 receptor sequences found in mammals.

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Additional file 5:

Hydropathy plots. Hydropathy plots and degree of conservation of GPRC5 consensus sequences from different species.

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Additional file 6:

Sequence analysis of GPRC5 receptors. (A) Alignment of the first extracellular loops from different classes of GPRC5 receptors. (B) Putative IQ motifs in GPRC5 receptors.

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Additional file 7:

Negative selection sites in GPRC5 receptors. GPRC5A and GPRC5D sequences from Mus musculus with marked sites where negative selection was predicted.

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Additional file 8:

mRNA sequences of Xenopus laevis sequences. Alignment of available mRNA sequences from XRAIG4 and GPRC5 receptors from Xenopus laevis.

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Open Data

Additional file 9:

GPRC5D from dolphin. Alignment of GPRC5D from Tursiops truncatus with the consensus of all mammalian GPRC5D sequences.

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Additional file 10:

GPRC5alignment.nex. Alignment of all sequences included in the study.

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Additional file 11:

alignment (Figure 3).nex. Alignment used to generate Figure 3A.

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Additional file 12:

Additional Materials. Figure legends of additional files, list of accession numbers of sequences included in this study, qPCR information.

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