Inversion analysis of the genomic region encompassing OTOP1. Three-color interphase FISH using probes WIBR2-1849E16 (red), WIBR2-1416B12 (blue), and WIBR2-1634L14 (green) was used to determine the orientation of the OTOP1-containing region in the human (HSA), chimpanzee (PTR: Marcus, Cochise, Douglas, Katie, and Veronica), orangutan (PPY; PPY9, PPY6, and Susie), and macaque (MMU) genomes. The inversion changes the order of the red and blue probes (mapping inside of the inversion) and their relative position with respect to the green probe (mapping outside of the inversion). FISH results show inversion of the region in human and chimpanzee with respect to orangutan and macaque.
Hurle et al. BMC Evolutionary Biology 2011 11:23 doi:10.1186/1471-2148-11-23