Alignment of CEN4 for all strains analysed. Centromeres are made up of two binding sites (CDEI and CDEIII) that are separated by an 87 bp CDEII. This CDEII region shows levels of polymorphism (θW = 0.04) that are typical of CDEII in general, and many more point substitutions (N = 16) than would be expected for a transposable element fragment of the same length (N = 3). This alignment is unambiguous and so shows that the high rates of mutation at centromeres are not the result of alignment error or slippage mutations.
Bensasson BMC Evolutionary Biology 2011 11:211 doi:10.1186/1471-2148-11-211