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Open Access Highly Accessed Research article

Evolutionary changes in the notochord genetic toolkit: a comparative analysis of notochord genes in the ascidian Ciona and the larvacean Oikopleura

Jamie E Kugler1, Pierre Kerner1, Jean-Marie Bouquet2, Di Jiang2 and Anna Di Gregorio1*

Author Affiliations

1 Department of Cell and Developmental Biology Weill Medical College of Cornell University 1300 York Avenue, Box 60, New York, NY 10065, USA

2 Sars International Centre for Marine Molecular Biology Thormøhlensgt. 55 N-5008 Bergen, Norway

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BMC Evolutionary Biology 2011, 11:21  doi:10.1186/1471-2148-11-21

Published: 20 January 2011

Additional files

Additional file 1:

Table 1. Ci-Bra target notochord genes and their Oikopleura counterparts found by the reciprocal best BLAST hit method.

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Additional file 2:

Figure S1. Protein sequences used for phylogenetic reconstructions.

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Additional file 3:

Table 2. Sequences of the oligonucleotides used as primers in the PCR amplifications.

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Additional file 4:

Table 3. Summary of the in situ hybridization results.

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Additional file 5:

Figure S2. Whole-mount in situ hybridization of Oikopleura neurulae.

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Additional file 6:

Figure S3. Additional expression patterns in Oikopleura embryos at 7-8 and 10-12 hpf.

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