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Open Access Research article

The genome sequence of Brucella pinnipedialis B2/94 sheds light on the evolutionary history of the genus Brucella

Stéphane Audic123*, Magali Lescot1, Jean-Michel Claverie1, Axel Cloeckaert4 and Michel S Zygmunt4*

Author Affiliations

1 Laboratoire Information Génomique et Structurale, CNRS-UPR2589, Aix-Marseille University, Institut de Microbiologie de la Méditerranée (IM2, IFR-88), Parc Scientifique de Luminy-163 Avenue de Luminy-Case 934-FR-13288, Marseille cedex 09, France

2 CNRS, UMR 7144, Equipe Evolution du Plancton et Paléo-Océans, Station Biologique de Roscoff, 29682 Roscoff, France

3 UPMC Univ Paris 06, UMR 7144, Adaptation et Diversité en Milieu Marin, Station Biologique de Roscoff, 29682 Roscoff, France

4 INRA, UR1282, Infectiologie Animale et Santé Publique, IASP, Nouzilly, F-37380, France

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BMC Evolutionary Biology 2011, 11:200  doi:10.1186/1471-2148-11-200

Published: 11 July 2011

Abstract

Background

Since the discovery of the Malta fever agent, Brucella melitensis, in the 19th century, six terrestrial mammal-associated Brucella species were recognized over the next century. More recently the number of novel Brucella species has increased and among them, isolation of species B. pinnipedialis and B. ceti from marine mammals raised many questions about their origin as well as on the evolutionary history of the whole genus.

Results

We report here on the first complete genome sequence of a Brucella strain isolated from marine mammals, Brucella pinnipedialis strain B2/94. A whole gene-based phylogenetic analysis shows that five main groups of host-associated Brucella species rapidly diverged from a likely free-living ancestor close to the recently isolated B. microti. However, this tree lacks the resolution required to resolve the order of divergence of those groups. Comparative analyses focusing on a) genome segments unshared between B. microti and B. pinnipedialis, b) gene deletion/fusion events and c) positions and numbers of Brucella specific IS711 elements in the available Brucella genomes provided enough information to propose a branching order for those five groups.

Conclusions

In this study, it appears that the closest relatives of marine mammal Brucella sp. are B. ovis and Brucella sp. NVSL 07-0026 isolated from a baboon, followed by B. melitensis and B. abortus strains, and finally the group consisting of B. suis strains, including B. canis and the group consisting of the single B. neotomae species. We were not able, however, to resolve the order of divergence of the two latter groups.

Keywords:
Brucella; Bacterial Genome Evolution; Comparative Genomics