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Open Access Highly Accessed Research article

Multigenic phylogeny and analysis of tree incongruences in Triticeae (Poaceae)

Juan S Escobar125*, Céline Scornavacca236, Alberto Cenci147, Claire Guilhaumon4, Sylvain Santoni4, Emmanuel JP Douzery2, Vincent Ranwez2, Sylvain Glémin2 and Jacques David4

Author affiliations

1 Institut National de la Recherche Agronomique, Centre de Montpellier, UMR Diversité et Adaptation des Plantes Cultivées, Domaine de Melgueil, 34130 Mauguio, France

2 Institut des Sciences de l'Evolution, UMR 5554 CNRS, Université Montpellier II, Place Eugène Bataillon, 34095 Montpellier Cedex 5, France

3 Laboratoire d'Informatique, de Robotique et de Microélectronique de Montpellier, UMR 5506, Université Montpellier II, 161 rue Ada, 34392 Montpellier Cedex 5, France

4 Montpellier Supagro, Centre International d'Etudes Supérieures en Sciences Agronomiques, UMR Diversité et Adaptation des Plantes Cultivées, 2 Place Pierre Viala, 34060 Montpellier Cedex 1, France

5 Department of Ecology and Evolutionary Biology, University of Toronto, 25 Willcocks Street, M5S 3B2 Toronto, Canada

6 University of Tübingen, Faculty of Computer Science, Chair of Algorithms in Bioinformatics, Sand 14, 72076 Tübingen, Germany

7 Institut de Recherche pour le Développement, UMR RPB-Equipe DIVA, 911 Avenue Agropolis, 34394 Montpellier Cedex 5, France

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Citation and License

BMC Evolutionary Biology 2011, 11:181  doi:10.1186/1471-2148-11-181

Published: 24 June 2011

Abstract

Background

Introgressive events (e.g., hybridization, gene flow, horizontal gene transfer) and incomplete lineage sorting of ancestral polymorphisms are a challenge for phylogenetic analyses since different genes may exhibit conflicting genealogical histories. Grasses of the Triticeae tribe provide a particularly striking example of incongruence among gene trees. Previous phylogenies, mostly inferred with one gene, are in conflict for several taxon positions. Therefore, obtaining a resolved picture of relationships among genera and species of this tribe has been a challenging task. Here, we obtain the most comprehensive molecular dataset to date in Triticeae, including one chloroplastic and 26 nuclear genes. We aim to test whether it is possible to infer phylogenetic relationships in the face of (potentially) large-scale introgressive events and/or incomplete lineage sorting; to identify parts of the evolutionary history that have not evolved in a tree-like manner; and to decipher the biological causes of gene-tree conflicts in this tribe.

Results

We obtain resolved phylogenetic hypotheses using the supermatrix and Bayesian Concordance Factors (BCF) approaches despite numerous incongruences among gene trees. These phylogenies suggest the existence of 4-5 major clades within Triticeae, with Psathyrostachys and Hordeum being the deepest genera. In addition, we construct a multigenic network that highlights parts of the Triticeae history that have not evolved in a tree-like manner. Dasypyrum, Heteranthelium and genera of clade V, grouping Secale, Taeniatherum, Triticum and Aegilops, have evolved in a reticulated manner. Their relationships are thus better represented by the multigenic network than by the supermatrix or BCF trees. Noteworthy, we demonstrate that gene-tree incongruences increase with genetic distance and are greater in telomeric than centromeric genes. Together, our results suggest that recombination is the main factor decoupling gene trees from multigenic trees.

Conclusions

Our study is the first to propose a comprehensive, multigenic phylogeny of Triticeae. It clarifies several aspects of the relationships among genera and species of this tribe, and pinpoints biological groups with likely reticulate evolution. Importantly, this study extends previous results obtained in Drosophila by demonstrating that recombination can exacerbate gene-tree conflicts in phylogenetic reconstructions.