Additional file 2.

Figure S2. Bar graphs of random distributions generated by the shuffle option in Mesquite using the Linear Parsimony Method. (A) Data showing presence of phylogenetic signal. The number of parsimony character steps for the reference tree (see Figure 6A) was significantly smaller, i.e. fell on the left side of the distribution, than the number of parsimony character steps for the trees with reshuffled taxa. (B) Data exhibiting lack of phylogenetic signal, i.e. random association between CHCs and the phylogeny (see Figure 6B) where the number of parsimony character steps for the reference tree fell within the 95% confidence interval. If the parsimony character steps for the reference tree fell on the extreme right of the distribution (not observed with our data) that would imply that CHC distributions were less conserved than by chance alone (e. g. due to character displacement). Vertical red lines represent 95% confidence intervals and green lines denote the mean number of parsimony character steps for the trees with reshuffled taxa. Red arrows represent the parsimony character steps for the reference tree.

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Oliveira et al. BMC Evolutionary Biology 2011 11:179   doi:10.1186/1471-2148-11-179