Table 3

Genetic diversity at four sites inhabited by Pyura praeputialis, and tests for genetic structure among them

Marker

Statistic

Sampling site


1

Fingal

2

Cape Conran

3

Kilcunda

4

Antofagasta


COI

h

0.968

± 0.028

0.965

± 0.024

0.835

± 0.037

0.971

± 0.024

π

0.007

± 0.004

0.006

± 0.003

0.005

± 0.003

0.007

± 0.004

ΦST

1

2

0.001

3

0.060*

0.103**

4

-0.024

0.005

0.087**

ANT

h

0.949

(± 0.017)

0.849

(± 0.033)

0.864

(± 0.041)

0.904

(± 0.023)

π

0.014

(± 0.009)

0.009

(± 0.006)

0.011

(± 0.007)

0.013

(± 0.008)

Hobs

0.153

(± 0.141)

0.214

(± 0.151)

0.156

(± 0.151)

0.205

(± 0.146)

Hexp

0.197

(± 0.186)

0.305

(± 0.162)

0.161

(± 0.149)

0.245

(± 0.178)

FST

1

2

-0.002

3

0.085**

0.044*

4

-0.004

0.007

0.061*


Site numbers refer to: 1 - Fingal (northern east coast of Australia); 2 - Cape Conran (southern east coast of Australia, east of the former Bassian Isthmus); 3 - Kilcunda (southeastern Australia, west of the former Bassian Isthmus); 4 - Antofagasta (Chile). Number of sequences generated for each site and genetic marker: Fingal: COI = 20, ANT = 38; Cape Conran: COI = 21, ANT = 28; Kilcunda: COI = 30, ANT = 30; Antofagasta: COI = 21 (including 6 sequences from Castilla et al. [34]), ANT = 52. Statistics include: h - haplotype or gene diversity; π - nucleotide diversity; ΦST - pairwise fixation index among sites based on mtDNA COI haplotypes; FST - pairwise fixation index among sites based on nrDNA ANT alleles; Hobs and Hexp - observed and expected mean heterozygosity based on ANT sequence data. Asterisks indicate significant fixation indices (*P < 0.05, **P < 0.01) and values in brackets are standard deviations.

Teske et al. BMC Evolutionary Biology 2011 11:176   doi:10.1186/1471-2148-11-176

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