Additional file 8.

IGH locus diagram with location of the VH genes from O. latipes. All genes were localized as indicated in material and methods (top left). Alignment of aminoacid sequences deduced from VH genes are at the bottom. The alignment was performed and scored according to recommendations of the IMGT. Naming was done using nomenclature proposed by the same organization. Phylogenetic tree of the VH regions from O. latipes is shown on the top right hand side. MEGA4 software, minimum evolution algorithm and JTT matrix were used to draw the tree. Differences by site are activated with gamma parameter 2.5.

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Magadán-Mompó et al. BMC Evolutionary Biology 2011 11:165   doi:10.1186/1471-2148-11-165