PCR products and Southern blot probed to 5S rDNA of stingrays. (a) PCR amplicons of 5S rDNA repeats from freshwater stingrays submitted to garose gel electrophoresis. 1-3, Potamotrygon motoro; 4-6, Potamotrygon falkneri; 7, Paratrygon aiereba. (b) Southern blot hybridization of HindIII digested genomic DNA of P. falkneri (1), P. motoro (2 and 3) and P. aiereba (4) probed with 5S rDNA class I of P. motoro. Bands of distinct molecular weight correspond to monomeric, dimeric, trimeric and tetrameric units, indicating sequence variants lacking the HindIII restriction site or undigested products. M, molecular marker with base pair sizes showed on the left.
Pinhal et al. BMC Evolutionary Biology 2011 11:151 doi:10.1186/1471-2148-11-151