Table 3

Expression changes in genes with swapped mutants and wild type.

SWAP

BY(WT)


Systemic name

TFBS

ΔCt Target/Act1

STDEV

ΔCt Target/Act1

STDEV

Nucleosome

occupancy


YBR248C

ABF1

3.091

0.167

2.443

0.070

NDR*

YDR519W

HAC1

3.648

0.012

2.846

0.050

NDR

YGL253W

RGT1

-0.259

0.179

-0.192

0.026

NDR

YLR214W

RCS1

3.245

0.270

2.747

0.079

NDR*

YLR450W

ROX1

1.757

0.236

1.688

0.031

NDR

YPL111W

ABF1

0.245

0.013

0.075

0.056

NDR*

YAL054C

CAT8

6.233

0.225

6.145

0.014

NR

YDR072C

PDR3

5.574

0.024

5.363

0.001

NR

YGL255W

ZAP1

7.008

0.048

5.435

0.004

NR*

YKL175W

ZAP1

2.685

0.184

2.788

0.013

NR

YML075C

ROX1

1.871

0.087

1.732

0.099

NR

YNL117W

CAT8

-1.444

0.056

-1.331

0.126

NR


Twelve genes that experienced TFBS gain or loss events at both NDRs and NRs were selected for site-directed mutagenesis and expression analysis. T-test was based on ΔCt of swapped mutants and BY4741(wild type).

* Significantly different from ΔCtBY(p-value< 0.05) by t-test

Swamy et al. BMC Evolutionary Biology 2011 11:150   doi:10.1186/1471-2148-11-150

Open Data