Figure 3.

Dose response curves of corticosteroid receptors. (A-E) Dose response curves characterized the sensitivity of mouse and midshipman MRs and GRs for aldosterone, 11-deoxycorticosterone (DOC), cortisol, and dexamethasone (see legend, lower left). The MR LBD constructs of both (A) mouse and (B) midshipman were sensitive to DOC, a precursor in the aldosterone biosynthetic pathway. (C) The mouse GR LBD was activated by cortisol, dexamethasone, and very high doses of aldosterone (> 10^-7 M), yet did not show a response to DOC. (D) Midshipman GR1 LBD showed very strong glucocorticoid specificity, while (E) the GR2 LBD showed low activation by all the corticosteroids at even very low doses, with increased activity at higher doses of dexamethasone and cortisol.