Table 1

Genetic diversity estimated using DNASP for each locus: Hd, haplotype diversity; S, number of segregating sites and π, nucleotide diversity.

Locus

Nbp/Nvar

Population

Nalleles

θ (from S)

Nhaplotype

Hd

S

π

Fu's Fs/R2


ND2

1041/170

debilis sensu lato

110

0.03106

43

0.948

170

0.05776

11.200/0.1757


albigula (Nguru + Usambara Mts)

59

0.00539

13

0.844

25

0.00586

1.303/0.1175


Nguru Mts

38

0.00161

6

0.751

7

0.00215

1.120/0.0839


Usambara Mts

21

0.00375

7

0.562

14

0.00239

-0.458/0.1553


Lowland (rabai + debilis sensu stricto)

51

0.02034

30

0.965

95

0.02068

-1.521//0.1103


rabai

41

0.01216

22

0.948

54

0.00833

-3.976**/0.0740


debilis sensu stricto

10

0.00579

8

0.956

17

0.00681

-1.104/0.1827


FGB

559/17

debilis sensu lato

184

0.00525

17

0.575

17

0.00432

-3.383*/0.0706


albigula (Nguru + Usambara Mts)

118

0.00067

3

0.05

2

0.00009

-3.527*/0.0466*


Nguru Mts

76

0.00036

2

0.052

1

0.00009

-1.248/0.0260**


Usambara Mts

42

0.00042

2

0.048

1

0.00009

-1.149/0.1525


Lowland (rabai + debilis sensu stricto)

66

0.00564

15

0.777

15

0.00460

-4.127**/0.0852


rabai

58

0.00425

10

0.713

11

0.00401

-1.198/0.1018


debilis sensu stricto

8

0.00483

6

0.929

7

0.00530

-1.637/0.1866


GAPDH

328/15

debilis sensu lato

208

0.00776

20

0.827

15

0.00622

-7.122**/0.0676


albigula (Nguru + Usambara Mts)

110

0.00464

11

0.824

8

0.00619

-1.261/0.1266


Nguru Mts

68

0.00511

10

0.824

8

0.00683

-0.934/0.1397


Usambara Mts

42

0.00355

6

0.661

5

0.00329

-1.016/0.1074


Lowland (rabai + debilis sensu stricto)

98

0.00771

16

0.556

13

0.00351

-10.918**/0.0439


rabai

70

0.00762

15

0.634

12

0.00443

-8.485**/0.0596


debilis sensu stricto

28

0.00157

3

0.320

2

0.00103

-0.731/0.0840


BRM

364/12

debilis sensu lato

168

0.00530

14

0.849

11

0.00768

-0.914/0.1257


albigula (Nguru + Usambara Mts)

96

0.00214

4

0.639

4

0.00226

0.852/0.1100


Nguru Mts

62

0.00264

4

0.633

4

0.00264

0.947/0.1284


Usambara Mts

34

0.00067

2

0.428

1

0.00118

1.407/0.2139


Lowland (rabai + debilis sensu stricto)

72

0.00397

10

0.815

7

0.00511

-1.598/0.1328


rabai

58

0.00415

9

0.759

7

0.00508

-1.221/0.1321


debilis sensu stricto

14

0.00259

4

0.692

3

0.00353

0.135/0.2143


In the absence of selection, significant negative values of Fu's Fs and low values of R2 are indicative of population expansion (*p < 0.05, **p < 0.01). For the three nuclear loci, only alleles with phase probabilities greater than 0.75 were included in the analyses. The subspecies rabai includes all individuals from lowland Tanzania (including the three specimens from SE Tanzania, whereas subspecies debilis only includes individuals from Mozambique and Zimbabwe, see text for details).

Fuchs et al. BMC Evolutionary Biology 2011 11:117   doi:10.1186/1471-2148-11-117

Open Data