Open Access Research article

Natural history of SLC11 genes in vertebrates: tales from the fish world

João V Neves1, Jonathan M Wilson2, Heiner Kuhl3, Richard Reinhardt3, L Filipe C Castro2 and Pedro NS Rodrigues14*

Author Affiliations

1 Iron and Innate Immunity, Instituto de Biologia Molecular e Celular (IBMC), Rua do Campo Alegre 823, 4150-180 Porto, Portugal

2 Centro Interdisciplinar de Investigação Marinha e Ambiental (CIIMAR), Rua dos Bragas 289, 4050-123 Porto, Portugal

3 Max-Planck-Institute for Molecular Genetics, Ihnestraße 63-73, 14195 Berlin, Germany

4 Instituto de Ciências Biomédicas Abel Salazar (ICBAS), Universidade do Porto, Largo Prof. Abel Salazar 2, 4099-003 Porto, Portugal

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BMC Evolutionary Biology 2011, 11:106  doi:10.1186/1471-2148-11-106

Published: 18 April 2011

Additional files

Additional File 1:

Figure S1: DNA and predicted amino acid sequence of sea bass slc11a2 isoforms. This file contains the cDNA sequences, as well as the putative proteins and characteristic features for each slc11a2 isoform.

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Additional file 2:

Figure S2: Comparative view of the genomic structure, organization and size of SLC11 homologs of several species. This file contains a comparative view of the genomic structure and size of sea bass slc11a2-α and slc11a2-β with homologs from other fishes, amphibians, mammals and insects.

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Additional file 3:

Figure S3: Additional phylogenetic trees. This file contains additional phylogenetic trees constructed with Bayesian, neighbour-joining and maximum-parsimony methods.

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Additional file 4:

Figure S4: Evolutionary relationships of AGAP2, ANKRD52 and MARCH9 gene families. This file contains phylogenetic trees for AGAP2, ANKRD52 and MARCH9, constructed with the maximum-likelihood method.

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Additional file 5:

Figure S5: Sea bass mortality during experimental infection. This file contains a graphic showing sea bass mortality during infection with Photobacterium damselae, at 12 hour intervals.

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Additional file 6:

Table S1: Primers used in this study. This file contains a table with all the primers used for sequencing, southern blot, in situ hybridization and gene expression analysis performed in this study.

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