Additional file 1.

PCR primers, and sequences used for Multipipmaker analysis. Top Left: A list of tools and databases utilized by the GRASP gene annotation pipeline. Top Right: PCR primers used for LWS opsin-positive X. helleri BAC clones, and for PCR screening of X. helleri adult female and male whole eye cDNA. PCR primers from other studies are referenced in "References" column. PCR results and sequencing notes for cDNA PCR screening are also listed in their respective columns. Bottom: Sequences from six teleost species used in addition to those described in this study for X. helleri for multipipmaker analyses. For Pundamilia pundamilia a GenBank accession number is provided. For all sequences used from one of the Ensembl genome browsers, assembly versions, chromosome/scaffold numbers and base pair positions are listed [100-108].

Format: PDF Size: 486KB Download file

This file can be viewed with: Adobe Acrobat Reader

Watson et al. BMC Evolutionary Biology 2010 10:87   doi:10.1186/1471-2148-10-87