Table 2

Amplification of shared single copy nuclear genes in Brassicaceae

Marker

At2 g21870

At2 g32520

At3 g47810

At4 g31720

At4 g33250

At5 g47570

At5 g63135

At5 g63135

At2 g13360

At4 g15790

At4 g37830

At5 g23290


RT-PCR


Brassica rapa

1

1

1

1

1

1

1

1

1

0

1

1


Brassica oleracea

1

1

1

1

1

1

1

1

1

0

1

1


Moricandria arvensis

1

1

1

1

1

1

1

1

1

0

1

1


Brassica repanda

1

1

1

1

1

1

1

1

1

1

1

1


Erucastrum canariense

1

1

1

1

1

1

0

1

1

1

0

1


Schouwia thebaica

1

1

1

1

1

1

0

1

1

1

0

1


Sisymbrium irio

1

1

1

1

1

1

1

1

1

0

1

1


Arabidopsis thaliana

1

1

1

1

1

1

0

1

1

0

1

1


Olimarabidopsis pumila

1

1

1

1

1

1

0

1

1

0

1

1


Chorispora tenella

1

1

1

1

1

1

0

1

1

0

1

1


Athionema saxatile

1

1

1

1

1

1

0

1

1

1

0

1


Medicago truncatula

1

1

1

1

1

1

0

1

1

1

0

1


Band Size in bp

450

450

350

250

210

300

250

300

900

150

150

150


PCR


Brassica rapa

3

1

1

3

3

3

2

1

1

1

1

2


Brassica oleracea

2

1

1

2

2

2

1

1

1

2

1

1


Sisymbrium irio

1

1

0

1

1

1

1

1

1

1

1

2


Arabidopsis thaliana

1

1

1

1

1

2

1

1

1

1

1

2


Olimarabidopsis pumila

1

1

0

1

2

1

2

1

1

1

1

2


Band Sizes in bp

700

1000

2000

1300

1200

2000

2000

3000

500

1000

1500

700

800

600

600

1100

700

400

300

200

500

700

200


PCR and RT-PCR amplification results from Brassicaceae showing number and size of bands. The number of bands amplified is indicated (0-5).

Duarte et al. BMC Evolutionary Biology 2010 10:61   doi:10.1186/1471-2148-10-61

Open Data