Table 3 |
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|
Standard set of 24 short PCR primer pairs for lophiiforms |
|||
|
No. |
Primer a |
Sequence (5' to 3') |
Reference |
|
|
|||
|
1 |
L1083-12S |
ACAAACTGGGATTAGATAC |
[47] |
|
H2590-16S |
ACAAGTGATTGCGCTACCTT |
[47] |
|
|
2 |
L2949-16S |
GGGATAACAGCGCAATC |
[47] |
|
H3976-ND1 |
ATGTTGGCGTATTCKGCKAGGAA |
[43] |
|
|
3 |
L2949-16S |
GGGATAACAGCGCAATC |
[47] |
|
H4432-Met |
TTTAACCGWCATGTTCGGGGTATG |
[46] |
|
|
4 |
L4299-Ile |
AAGGRCCACTTTGATAGAGT |
This study |
|
H5669-Asn |
AACTGAGAGTTTGWAGGATCGAGGCC |
[53] |
|
|
5 |
L4633-ND2 |
CACCACCCWCGAGCAGTTGA |
[47] |
|
H5669-Asn |
AACTGAGAGTTTGWAGGATCGAGGCC |
[53] |
|
|
6 |
L5549-Trp |
AAGACCAGGAGCCTTCAAAG |
This study |
|
H6558-CO1 |
CCKCCWGCKGGGTCAAAGAA |
[53] |
|
|
7 |
L6205-CO1 |
TTCCCWCGAATAAATAACATAAG |
[87] |
|
H7447-Ser |
AWGGGGGTTCRATTCCTYCCTTTCTC |
[87] |
|
|
8 |
L7255-CO1 |
GATGCCTACACMCTGTGAAA |
[47] |
|
H8312-Lys |
CACCWGTTTTTGGCTTAAAAGGCTAAYGCT |
[87] |
|
|
9 |
L8202-CO2 |
TGYGGAGCWAATCAYAGCTT |
[87] |
|
H9375-CO3 |
CGGATRATGTCTCGTCATCA |
[53] |
|
|
10 |
L8343-Lys |
AGCGTTGGCCTTTTAAGCTAAWGATWGGTG |
[87] |
|
H9639-CO3 |
CTGTGGTGAGCYCAKGT |
[47] |
|
|
11 |
L8343-Lys |
AGCGTTGGCCTTTTAAGCTAAWGATWGGTG |
[87] |
|
H10019-Gly |
CAAGACKGKGTGATTGGAAG |
[47] |
|
|
12 |
L8343-Lys |
AGCGTTGGCCTTTTAAGCTAAWGATWGGTG |
[87] |
|
H10433-Arg |
AACCATGGWTTTTTGAGCCGAAAT |
[47] |
|
|
13 |
L10054-Gly |
CACCWGGTCTTGGTTWAAMTCCMAGGAAAG |
This study |
|
H11534-ND4M |
GCTAGKGTAATAAWKGGGTA |
[87] |
|
|
14 |
L10440-Arg |
AAGATTWTTGATTTCGGCT |
[27] |
|
H11534-ND4M |
GCTAGKGTAATAAWKGGGTA |
[87] |
|
|
15 |
L11424-ND4 |
TGACTTCCWAAAGCCCATGTAGA |
[47] |
|
H12632-ND5 |
GATCAGGTTACGTAKAGKGC |
[47] |
|
|
16 |
L12329-Leu |
CTCTTGGTGCAAMTCCAAGT |
[47] |
|
H13396-ND5 |
CCTATTTTTCGGATGTCTTG |
[53] |
|
|
17 |
L12329-Leu |
CTCTTGGTGCAAMTCCAAGT |
[47] |
|
H13727-ND5 |
GCGATKATGCTTCCTCAGGC |
[47] |
|
|
18 |
L13553-ND5 |
AACACMTCTTAYCTWAACGC |
[53] |
|
H14768-CYB |
TTKGCGATTTTWAGKAGGGGGTG |
[87] |
|
|
19 |
L13553-ND5 |
AACACMTCTTAYCTWAACGC |
[53] |
|
H15149-CYB |
GGTGGCKCCTCAGAAGGACATTTGKCCTCA |
[53] |
|
|
20 |
L14504-ND6 |
GCCAAWGCTGCWGAATAMGCAAA |
[53] |
|
H15560-CYB |
TAGGCRAATAGGAARTATCA |
[47] |
|
|
21 |
L14718-Glu |
TTTTTGTAGTTGAATWACAACGGT |
This study |
|
H15913-Thr |
CCGGTSTTCGGMTTACAAGACCG |
[87] |
|
|
22 |
L15369-CYB |
ACAGGMTCAAAYAACCC |
[53] |
|
H16484-CR |
GAGCCAAATGCMAGGAATARWTCA |
[87] |
|
|
23 |
L15998-Pro |
AACTCTTACCMTTGGCTCCCAARGC |
[53] |
|
H885-12S |
TAACCGCGGYGGCTGGCACGA |
[87] |
|
|
24 |
L16507-CR |
TGAWYTATTCCTGGCATTTGGYTC |
[87] |
|
H1358-12S |
CGACGGCGGTATATAGGC |
[47] |
|
|
|
|||
|
a L and H denote light and heavy strands, respectively. Positions with mixed bases are labeled with their IUB codes |
|||
|
Miya et al. BMC Evolutionary Biology 2010 10:58 doi:10.1186/1471-2148-10-58 |
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