Additional file 9.

wg, Wnt5, Wnt11 and Wnt16 expression in Achaearanea, and WntA expression in Cupiennius. At-wg expression is first detected at stripes in L1 and L2 (a). By stage 9, At-wg is expressed in anteroventral regions of the prosomal limb buds and dots in the dorsal of O2 and O3, but no expression is seen in the other opisthosomal segments or in the SAZ (b). Later at stage 10, At-wg is expressed as stripes in O2 and O3 and expression is also observed in the labrum and the hindgut (c). At-Wnt5 expression is first observed in an anterior stripe at stage 5 that broadens during stage 6 (d). At-Wnt11-2 is first expressed at the posterior pole of the embryo during stage 6 (e). Similar to At-Wnt5, At-Wnt16 expression is observed as a broad anterior stripe at stage 6 (f). Strong expression of Cs-WntA is visible in the SAZ (g), (g'). Weaker expression of Cs-WntA is also detectable at the distal ends of the spinnerets (h), (h') and in two small spots in the cheliceres (i), (i') indicated by arrows. Lateral views are shown in (a), (b), (g) and (g'), ventral views with posterior wrapping to the right in (c), (d) and (f), posterior view with dorsal up in (e), ventral views with posterior to the right in (h) and (h'), and anterior views with posterior to the right in (i) and (i'). Brightfield and DAPI counterstained images of the same embryos are shown in (g), (h), (i) and (g'), (h'), (i') respectively. Ch, cheliceres; Lb, labrum; L1 and L4, leg bearing segments; O1 to O5, opisthosomal segments; SAZ, segment addition zone.

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Janssen et al. BMC Evolutionary Biology 2010 10:374   doi:10.1186/1471-2148-10-374