Additional file 10.

Additional expression patterns of Wnt ligand genes in the annelid Platynereis. (a)-(e) ventral views of 48 hpf trochophores. The black dashed line is the prototroch. Red arrowheads: Broad Pd-Wnt6 expression in the mesodermal bands (a) and in few cells of the anterior mesoderm for Pd-Wnt10 (d); Black hollow arrowhead: Pd-Wnt9 expression in the proctodeum; Black asterisk: Pd-WntA expression in the stomodeum; pX: Pd-WntA expression in the setal sacs. (f)-(j) details of Wnt gene expression during posterior growth. (f) Frontal optical section of a 7-day regenerate; Red arrowhead: striped Pd-Wnt5 expression in the mesoderm and ectoderm of forming segments. (g) Ventral view of a 7-day regenerate showing the mesodermal expression of Pd-Wnt6. (h) Transverse section in a nascent segment of a 7-day regenerate, showing isolated cells in the gut expressing Pd-Wnt9; g: gut lumen; end: gut endoderm; gm: gut mesoderm; dlm: dorsal longitudinal muscles; vlm: ventral longitudinal muscles; vnc: ventral nerve cord; p: parapodia. (i) Ventral view of a 7-day regenerate showing parapodial expression of Pd-WntA. (j) Close up dorsal view of nascent segments in a 7-day regenerate, showing Pd-WntA expression in the walls of lateral vessels (red arrowheads) branching from the dorsal longitudinal vessel (dlv). The yellow dashed line in (e), (g), (i) is the approximate position of the SAZ.

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Janssen et al. BMC Evolutionary Biology 2010 10:374   doi:10.1186/1471-2148-10-374