Additional file 2.

UPGMA-tree based on mitochondrial hypervariable region 1 (HVR1). This file contains a UPGMA-tree based on sequences of hypervariable region 1. Calculation was done by using the average distance BLOSUM62 [21] routine in JALVIEW [22]. A total set of 377 argentatus from 16 different European colonies, 32 marinus from five European colonies and 32 marinus from three eastern North American colonies, 32 hyperboreus from four Eurasian colonies and 35 hyperboreus from four North American colonies, 35 smithsonianus from four North American colonies, 30 fuscus and graellsii, 31 michahellis and atlantis, and 33 cachinnans were sequenced on HVR1 (see also Additional files 1 and 3) and used for the tree calculation. Based on this resulting tree, all samples were assigned to either clade 1 or clade 2. Rooting of the tree was done by the use of previously published [8] Western gull (L. occidentalis) sequences.

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Sternkopf et al. BMC Evolutionary Biology 2010 10:348   doi:10.1186/1471-2148-10-348