Table 2 |
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|
Primers for real-time qPCR, amplification efficiency (%) and amplicon size (bp). |
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|
Gene |
Name |
Sequence (5' to 3') |
Efficiency |
Size |
|
|
||||
|
α1 |
A1F |
GACTTACTTCAGCCACTGGAAGAGCCT C |
96 |
153 |
|
A1R |
TTGAAGGCGTGCAGCTCGCTCAGAG |
|||
|
α2 |
A2F |
GTCCTATTTCTCTCACTGGAAGGACGCG |
85 |
153 |
|
A2R |
ATGAACGCGTGCAGCTCGCTAAGGC |
|||
|
α3 |
A3F |
CACATCATACCCTGGCACCAAGAC |
95 |
172 |
|
A3R |
CTGGTAGGCGTGGTAGGTTTGAAGAG |
|||
|
α4 |
A4F |
TTCTCCCACTGGAAAGACCTCGG |
70 |
138 |
|
A4R |
ATGGAGCTCACTGAGCTCGAGAAG |
|||
|
β1 allele A |
B1FA |
TTATGGGAAACCCCAAGGTGGCCAA |
91 |
131 |
|
B1R |
GTGCAGTTTCTCGGAGTGCAGCACGC |
|||
|
β1 allele B |
B1FB |
TTGTGGGAAACCCCAAGGTGGCTGC |
98 |
131 |
|
B1R |
GTGCAGTTTCTCGGAGTGCAGCACGC |
|||
|
β2 |
B2F |
CCTGTACAATGCAGAGACCATCATGGC |
84 |
151 |
|
B2R |
GTGCAGCTTGTCAGAGTGGAGCAGAG |
|||
|
β3 |
B3F |
ACAGATAGTGAGCGCGCCATCATTAA |
86 |
176 |
|
B34R |
GCGGCGATCAGGGGGTTGCACAG |
|||
|
β4 |
B4F |
ACAGATAGTGAGCGCGCCATCATTAC |
95 |
176 |
|
B34R |
GCGGCGATCAGGGGGTTGCACAG |
|||
|
β5 |
B5F |
GTGGACTCGGAGGTACTTTGGAAAC |
89 |
168 |
|
B5R |
TGCAGCTGACTGAGCTCGCAATAG |
|||
|
Ubiquitin |
UbiF |
GGCCGCAAAGATGCAGAT |
81 |
69 |
|
UbiR |
CTGGGCTCGACCTCAAGAGT |
|||
|
|
||||
|
Wetten et al. BMC Evolutionary Biology 2010 10:315 doi:10.1186/1471-2148-10-315 |
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