Table 2

Primer pairs and annealing temperatures successfully used for primer walking.

Primer Pair

Primer Sequence (3'-5')

Annealing Temperature (°C)


SR 16S-f

CTT ATG TTT TTT TTA GTT TGC GAC CTC

62

SR cob-f

GGG GGA GTG ATT GCT TTG TTG C

64

SR cox3-f

CAA CAG GGT TTC ACG GAA TAC ACG

65

SR 16S-r

GAG GTC GCA AAC TAA AAA AAA CAT AAG

62

SR cobf-rev

TGT TGG AGA TCT AAC AGA ATA AGC AC

60.1

SR co1f-rev

CTT TCT GAG ATA AAA GTA GGT CCT GG

61.6


Sequences are illustrated from 5' to 3' end.

Mwinyi et al. BMC Evolutionary Biology 2010 10:309   doi:10.1186/1471-2148-10-309

Open Data