Table 2 |
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|
Statistical results from experiments 2a-2c: fecundity and behavioural responses to alarm pheromone exposure. |
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|
Day 1 offspring |
Total offspring (Day 2-6) |
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|
|
|
|||||
|
2a: aphid line by EBF exposure |
d.f. |
F |
P |
d.f. |
F |
P |
|
|
|
|||||
|
Line |
3, 189 |
0.40 |
0.7500 |
3, 171 |
7.81 |
< 0.0001 |
|
Exposure |
1, 189 |
0.06 |
0.8000 |
1, 171 |
0.54 |
0.4600 |
|
Line*Exp |
3, 189 |
1.13 |
0.3400 |
3, 171 |
0.77 |
0.5100 |
|
Day 1 offspring |
Total offspring (Day 2-6) |
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|
|
|
|||||
|
2bi: symbiont by EBF exposure (5A) |
d.f. |
F |
P |
d.f. |
F |
P |
|
|
|
|||||
|
Symbiont |
3, 147 |
10.49 |
< 0.0001 |
3, 127 |
1.80 |
0.1500 |
|
Exposure |
1, 147 |
0.26 |
0.6100 |
1, 127 |
0.04 |
0.8400 |
|
Symb*Exp |
3, 147 |
6.22 |
0.0005 |
3, 127 |
0.17 |
0.9200 |
|
Day 1 offspring |
Total offspring (Day 2-6) |
|||||
|
|
|
|||||
|
2bii: symbiont by EBF exposure (LSR1) |
d.f. |
F |
P |
d.f. |
F |
P |
|
|
|
|||||
|
Symbiont |
1,76 |
11.03 |
0.0014 |
1,58 |
4.30 |
0.0430 |
|
Exposure |
1,76 |
0.65 |
0.4220 |
1,58 |
0.72 |
0.4000 |
|
Symb*Exp |
1,76 |
5.45 |
0.0220 |
1,58 |
2.86 |
0.0960 |
|
Day 1 offspring |
Total offspring (Day 2-6) |
|||||
|
|
|
|||||
|
2biii:line by symbiont by EBF exposure |
d.f. |
F |
P |
d.f. |
F |
P |
|
|
|
|||||
|
Line |
1, 151 |
2.44 |
0.1202 |
1, 128 |
0.53 |
0.4699 |
|
Symbiont |
1, 151 |
24.26 |
< 0.0001 |
1, 128 |
3.26 |
0.0732 |
|
Line*Symb |
1, 151 |
0.36 |
0.5495 |
1, 128 |
2.83 |
0.0947 |
|
Exposure |
1, 151 |
1.07 |
0.3015 |
1, 128 |
0.60 |
0.4417 |
|
Line*Exp |
1, 151 |
4.36 |
0.0384 |
1, 128 |
0.43 |
0.5135 |
|
Symb*Exp |
1, 151 |
0.005 |
0.7412 |
1, 128 |
0.94 |
0.3339 |
|
Line*Symb*Exp |
1, 151 |
9.69 |
0.0022 |
1, 128 |
3.52 |
0.0629 |
|
Proportion of time on plant |
Proportion of time active |
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|
|
|
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|
2c: symbiont by EBF behaviour (5A) |
d.f. |
χ2 |
P |
d.f. |
χ2 |
P |
|
|
|
|||||
|
Symbiont |
3 |
11.38 |
0.7463 |
3 |
25.15 |
0.0007 |
|
Exposure |
2 |
176.29 |
< 0.0001 |
2 |
97.38 |
< 0.0001 |
|
Symb*Exp |
6 |
106.13 |
0.0753 |
6 |
6.90 |
0.5825 |
|
|
||||||
|
ANOVA statistics of fecundity data for experiments where aphids from different clonal lines (2a: 5A, G3, G6, LSR1) or with different symbionts (2bi: no symbiont, Hamiltonella defensa, Regiella insecticola, Serratia symbiotica; 2bii: no symbiont, Regiella insecticola; 2biii: 5A and LSR1 with no symbiont, or Regiella insecticola) exposed to the alarm pheromone EBF or hexane as a control. (2c) GLM statistics of the proportion of time aphids were either on their plant, or active after exposure to EBF, hexane or water. |
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|
Barribeau et al. BMC Evolutionary Biology 2010 10:251 doi:10.1186/1471-2148-10-251 |
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