Experimental procedure with a special emphasize on the evolutionary processes that operate through generations. Bars represent the diploid genotypes present in a virtual population (Early or Late population derived from either F252 or MBS). White and black boxes indicate homozygous regions that are fixed for one of the allele (Early or Late). Heterozygosity can result either from standing variation present at G0 (grey box) or from new mutations occurring in the subsequent generations (white box with a star). Mutations from both sources can become fixed during the course of the experiment (G1 to G7) because of genetic drift and/or selection. At each generation, molecular polymorphism (as revealed by RFLP on 10 candidate regions) and phenotypic variation were evaluated in order to detect association between RFLP and flowering time variation.
Durand et al. BMC Evolutionary Biology 2010 10:2 doi:10.1186/1471-2148-10-2