Table 2

Primers used for amplification of GSII genes from green algae

Gene

Primer name

Direction

Sequence


MossGS2-1F

Forward

5'-TGGGTTGATGGTMANGARGG-3'

GSIIB

MossGS2-2R

Reverse

5'-ATNCCGAAMTCTTCNCC-3'

Green UNI 1-F

Forward

5'-CCIRAITGGWSITTYGAYGG-3'

cpGSII(QGPFY)-R

Reverse

5'-CCRCARTARAAIGGICCYTGIGG-3'


GALG GS F

Forward

5' - TGC CCA TCC CCA CCA ACA C - 3'

GSIIE

GALG GS R

Reverse

5' - TCT CGT GCT TGC CCG TCA GG - 3'

GS2ChloroF

Forward

5' - CGG CWT CGA GCA GGA GTA CAC - 3'

GS2ChloroR

Reverse

5' - CCG AYC TGG WAC TCC CAC TGG - 3'


Sequences of degenerate primers are presented using IUBMB single letter codes. I represents inosine.

Ghoshroy et al. BMC Evolutionary Biology 2010 10:198   doi:10.1186/1471-2148-10-198

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