Table 3

Query Sequences used to Perform BLAST Searches of the G. gallus and T. guttata genomes.

Feature

Lowest E-Value

(G. gallus/T. guttata)

Cut-off E-Value

(G. gallus/T. guttata)

Highest BLAST Score (G. gallus/T. guttata)

Cut-off BLAST score (G. gallus/T. guttata)

GI # or publication

Feather β-Keratin Coding (FK) [A,B,C,D]

1.00e-162/

7.00e-70

3.00e-95/2.00e-60

565/258

342/226

GI:62929 (A)

GI:62930 (B)

GI: 126165215 (C)

GI:62931 (D)

Feather-Like Coding (FL)

1.00e-155/5.00e-113

2.00e-93/6.00e-68

542/401

337/251

Presland et al [11]

Claw β-keratin Coding

6.00e-151/4.00e-53

4.00e-125/4.00e-53

528/202

442/202

GI:211431

Scale β-keratin Coding

5.00e-95/9.00e-57

1.00e-75/9.00e-54

339/211

274/201

GI:63548

Keratinocyte

0.0/NA

0.0/NA

743/NA

743/NA

GI:154800479

Beak β-keratin

NA/NA

NA/NA

NA/NA

NA/NA

GI:29570104

β-keratin in jun-transformed cells (BKJ)

1.00e-143/NA

1.00e-143/NA

511/NA

511/NA

GI:1016678

Query sequences used in BLAST searches and the NCBI GI number or publication where these sequences can be located. The lowest and highest (cut-off) E-value, the highest and lowest (cut-off) BLAST score for identification of β-keratin sequences on GGA25 and TGU25 are listed. These sequences were also used to search the entire genomes of both G. gallus and T. guttata using an E-value cut-off of 1e-10 for those searches involving additional feather β-keratins [7,8].

Greenwold and Sawyer BMC Evolutionary Biology 2010 10:148   doi:10.1186/1471-2148-10-148