Open Access Research article

The gene transformer-2 of Anastrepha fruit flies (Diptera, Tephritidae) and its evolution in insects

Francesca Sarno1, María F Ruiz1, José M Eirín-López2, André LP Perondini3, Denise Selivon3 and Lucas Sánchez1*

Author Affiliations

1 Centro de Investigaciones Biológicas (C.S.I.C.), Ramiro de Maeztu 9, 28040 Madrid, Spain

2 CHROMEVOL-XENOMAR Group, Departamento de Biología Celular y Molecular, Universidade da Coruña, 15071 A Coruña, Spain

3 Departamento de Genética e Biologia Evolutiva, Instituto de Biociências, Universidade de São Paulo, 05508-0900 Sao Paulo, Brazil

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BMC Evolutionary Biology 2010, 10:140  doi:10.1186/1471-2148-10-140

Published: 13 May 2010

Additional files

Additional file 1:

Number of nucleotides that compose the exons and introns of gene tra-2 of A. obliqua, C. capitata and D. melanogaster. Exons (boxes) and introns (lines) are not drawn to scale.

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Additional file 2:

Percentage similarity among the Anastrepha Tra2 proteins. Comparison of the Tra2 proteins of Anastrepha species.

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Additional file 3:

Percentage similarity among the insect Tra2 proteins. Comparison of the Tra2 proteins of the insects so far characterised. A. obliqua was used as the reference species for the Anastrepha species.

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Additional file 4:

Amino acid alignment of the Tra2 protein in the analysed species. The different protein domains are indicated as follows: RS-rich regions in grey background, RNA recognition motif (RRM) in green background, and linker region in yellow background. The RNP-1 and RNP-2 elements are indicated in open black boxes.

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Additional file 5:

Evolutionary parameters of the Tra2 proteins from insects. Average numbers of amino acid (pAA), total nucleotide (pNT), synonymous (pS) and non-synonymous (pN) nucleotide differences per site, codon bias (Effective Number of Codons, ENC) and Z-test of selection in complete tra-2 genes and independent tra-2 domains from insects. SE, standard error; R, average transition/transversion ratio; n/a, not applicable. a H0: pN = pS; H1: pN < pS.

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