Research article
The evolutionary diversification of LSF and Grainyhead transcription factors preceded the radiation of basal animal lineages
1 Department of Biology, Boston University, 5 Cummington Street, Boston, Massachusetts 02215, USA
2 Kewalo Marine Laboratory, Pacific Biomedical Research Center, University of Hawaii, 41 Ahui Street, Honolulu, HI 96813, USA
BMC Evolutionary Biology 2010, 10:101 doi:10.1186/1471-2148-10-101
Published: 18 April 2010Additional files
Additional file 1:
Output from the motif discovery program MEME.
Format: TXT Size: 422KB Download file
Additional file 2:
The tree shown is based on a maximum-likelihood analysis of the amino acids in the gap free alignment using the program RAxML[73]. Numbers at nodes represent bootstrap support. The tree is drawn as though rooted between the metazoan sequences and the fungal sequences. Branch length is shown in terms of expected number of substitutions per residue (bar at lower left).
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Additional file 3:
The assembled genomes ofChlamydomonas reinhardtii, Dictyostelium purpureum, and Selaginella moellendorffii (housed at the JGI Genome Portal [48]) were queried with a human LSF sequence (>gi|21361278|ref|NP_005644.2| transcription factor CP2) and GRH sequence (>gi|46854865|gb|AAH69633.1| GRHL2 protein). A permissive E value cut-off was specified (e-1). Dictyostelium and Chlamydomonas each returned a single hit for LSF, and Selaginella returned a single hit for GRH.
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Additional file 4:
All positions in the full alignment containing gaps were deleted to produce this gap-free alignment comprising 44 characters.
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