Additional file 5.

Figure S5: . Overview of Ipiwi1 expression and dynamics during posterior regeneration in Isodiametra pulchra. This whole mount overview clearly demonstrates that Ipiwi1 is only locally upregulated within the regeneration blastema (for details see Figure. 4). Expression of Ipiwi1 mRNA (A-I) and protein localization (J-R). One hour after cutting (A; J) and up to five hours postamputation (B, K) Ipiwi1 could not be detected at the regeneration site by in situ hybridization. Ipiwi1 was upregulated in the regeneration blastema (arrow) after 10 hours (C, L) and 25 hours postamputation (D, M). From 42 hours onwards Ipiwi1 remained downregulated in the regeneration blastema (E-R) and Ipiwi1 expression and protein were only present in the differentiating genital blastema (open arrows in E-Q). Scale bars 100 μm.

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De Mulder et al. BMC Developmental Biology 2009 9:69   doi:10.1186/1471-213X-9-69