Additional file 4.

Figure S4: Ipiwi2 expression, Ipiwi1 and Ipiwi2 control sense probes, Ipiwi1 Western Blot and radiation controls. Ipiwi2 whole mount in situ hybridization (A) with detail of expression in testes (t) (B) and in developing eggs (de) (C). (D) Ipiwi1 sense control. (E) Ipiwi2 sense control. (F) Western blot of Ipiwi1 polyclonal antibody, showing a signal at the expected size (100 kDa). (G-I) Hard X ray radiation of 60 Gray did not result in a significant downregulation of the housekeeping gene Isodiametra pulchra elongation factor alpha (IpEfα). IpEfα Control (G); IpEfα expression after one day (H) and one week (I) postirradiation. Scale bars 100 μm in (A, D, E, G, H, I), 50 μm in (B) and 25 μm in (C).

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De Mulder et al. BMC Developmental Biology 2009 9:69   doi:10.1186/1471-213X-9-69